摘要
目的:建立小鼠脑内去甲肾上腺素含量的高效液相色谱-电化学(HPLC-ECD)测定方法。方法:小鼠脑组织采用70%高氯酸沉淀蛋白后,以HPLC-ECD法进行分析。使用汉邦lichrospher C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为醋酸钾缓冲液-甲醇(95:5,pH 4.5),流速为1.0 ml·min^(-1),温度为25℃;电化学检测器电压为0.6 V,电流为100 nA,频率为0.05 Hz。结果:去甲肾上腺素在0.1～25μmol·L^(-1)范围内线性关系良好,日内、日间RSD分别小于7.81%和3.61%,回收率为75.05%,测得小鼠脑内去甲肾上腺素含量为(8.94±0.71)μg·g^(-1)。结论:该方法快速、准确。
Objective：To develop an HPLC-ECD method to determine norepinephrine in mouse brain.Method：The supernatant of mouse brain tissue homogenate precipitated by 70%HClO_4 was analyzed by HPLC-ECD method.A Hanbon lichrospher C_（18） column was used in the separation.The mixture of potassium acetate buffer and methanol（95：5,pH 4.5） was used as the mobile phase.The flow rate was 1.0 ml · min^（-1）.The column temperature was 25℃.The detection voltage,current and frequency value was set at 0.6 V,100 nA and 0.05 Hz,respectively.Result：Norepinephrine had good linearity within the range of 0.1-25μmol· L^（-1）.Intraand inter-day RSD of the method was below 7.81%and 3.61%,respectively.The recovery of norepinephrine was 75.05%.The detection limit was 17 ng ？ ml^（-1）.The content of norepinephrine determined in mouse tissue was（8.94±0.71 ）μg ？ g^（-1）.Conclusion： The method is fast,simple and accurate in the determination of norepinephrine.
出处
《中国药师》
CAS
2012年第12期1682-1685,共4页
China Pharmacist
基金
国家科技重大专项(编号:2010ZX09401-306-1-1)
重庆科技创新能力建设项目(编号:CSTC
2010AA5058)
