摘要
目的:研究丹参酮ⅡA(TanⅡA)对人鼻咽癌CNE1细胞增殖及对共刺激分子B7H3表达的影响。方法:不同浓度TanⅡA作用人鼻咽癌CNE1细胞后,四甲基唑盐比色法检测CNE1细胞增殖,流式细胞术检测CNE1细胞凋亡和细胞周期,直接免疫萤光法检测CNE1细胞凋亡蛋白Bcl-2和共刺激分子B7H3的表达变化。结果:TanⅡA对CNE1细胞生长表现出明显抑制作用,抑制率与药物呈明显的时间与浓度依赖效应,差异有统计学意义(P<0.01);24h、48h、72h的IC50值分别为12.5μmol/L、4.8μmol/L和3.0μmol/L;低、中、高浓度组CNE1细胞凋亡率较对照组升高,差异有统计学意义(P<0.01);细胞周期阻滞于G2/M期,低、中、高浓度组Bcl-2及B7H3表达较对照组下调,差异有统计学意义(P<0.05)。结论:TanⅡA对CNE1细胞具有抑制生长、促进凋亡的作用,TanⅡA下调Bcl-2及B7H3的表达可能是抗肿瘤作用机制之一。
Objective：To explore the proliferation of human nasopharyngeal carcinoma CNE1 cell and B7H3 experation after treating by Tanshinone ⅡA（Tan ⅡA）.Method：The CNE1 cell was cultured in vitro and treated by Tan ⅡA with different concentrations;Cell proliferation was valuated by MTT;Apoptotic rate and cell cycle were investigated by flow cytometry;Bcl2 and B7H3 experation were detected by direct immunofluorescence assay.Result：The CNE1 cell′s proliferation has been significantly inhibited by Tan ⅡA,the inhibition was in dose and time effect with Tan ⅡA,with significant difference（P0.01）;IC50 value in 24,48 and 72 hours was respective 12.5 μmol/L,4.8 μmol/L and 3.0 μmol/L;Comparing with the control group,Tan ⅡA group increased apoptosis rate（P0.01）;Cell cycle was blocked in G2/M phase;Bcl2 and B7H3 expression were more decreased in Tan ⅡA group than in control group with significant difference（P0.05）.Conclusion：Tan ⅡA caused obviously growth inhibitory effect and induced apoptosis in CNE1 cells and the mechanisms might be related to down regulation of Bcl2 and B7H3.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2013年第4期199-202,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
关键词
鼻咽肿瘤
丹参酮ⅡA
增殖
B7H3
nasopharyngeal neoplasms
Tanshinone ⅡA
proliferation
B7H3
