摘要
目的采用改良的自由落体撞击法建立小鼠重型颅脑损伤后肠动力障碍（intestinalmotilitydisorders，IMD）模型，为进一步研究损伤后IMD机制奠定基础。方法采用自制圆柱套杆撞击器对小鼠致伤，于伤后1，6h、1，3，7d各时相点观察脑、肠组织的病理学改变，检测脑含水量、小肠推进率。结果重型颅脑损伤后在脑组织出血水肿时期，肠道亦有急性炎症、充血等表现；脑含水量于6h增加，24h达高峰；损伤后1h即出现小肠推进率的下降，6h达最低值。结论小鼠重型颅脑损伤后存在IMD，本装置采用改良Feeney自由落体撞击法可成功建立重型颅脑损伤后IMD的小鼠模型。
Objective To build model of intestinal motility disorders （IMD） in mice after severe craniocerebral trauma by using modified free fall impact method so as to lay the foundation for further study on development mechanism of postinjury IMD. Methods Severe eraniocerebral trauma in mice was induced by a cylindrical sleeve rod impactor. Pathological changes of cerebral and intestinal tissue in mice were observed at 1, 6 hours and 1, 3, 7 days after severe craniocerebral trauma. Brain water content and intestinal propulsion percentage were detected in each time point as well. Results Acute inflammation and congestion of the intestine were observed in phase of cerebral hemorrhage and edema after severe eraniocerebral trauma in mice. Brain water content was increased at 6 hours and reached the peak at 24 hours. Intestine propulsion percentage showed a fall at one hour and dropped to the lowest level at 6 hours. Conclusions IMD exists in mice with severe craniocerebral trauma. The device involving modified Feeney free fall impact method can successfully establish animal model of IMD in mice with severe craniocerebral trauma.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2013年第2期165-169,共5页
Chinese Journal of Trauma
基金
国家自然科学基金资助项目（81272079）
关键词
颅脑损伤
肠
动力学
模型
动物
Craniocerebral trauma
Intestines
Kinetics
Models, animal
