摘要
目的探讨BM．PRP制备的可行性并比较BM—PRP与PB-PRP中PLT数量的差异。方法成年新西兰白兔8只，BM组和PB组分别采用骨髓穿刺和耳中央动脉穿刺抽取标本各10ml／只（含1．5m14．7％的枸橼酸钠作为抗凝剂），采用二次离心的方法制备新西兰白兔的BM—PRP和PB—PRP，手工计数其中的血小板数量，采用t检验进行统计学分析。结果BM-PRP中的的PLT数量（2296．76±148．80）X10^／μl是PB—PRP中PLT浓度（1633．26±89．64）×10^3／μl的1．41倍（P〈0．001）。结论骨髓穿刺是获取PRP的一种有效方法，并可获得较PB—PRP更高浓度的PRP。
Objective To discuss the preparation of bone marrow-derived platelet-rich plasma （BM- PRP） and to compare the differences in the number of platelets between BM-PRP and peripheral blood derived platelet-rich plasma （PB-PRP）. Methods 10 ml （ containing 1.5 ml 4.7% sodium citrate as an anticoagulant） of BM were obtained using bone marrow aspirate techniques and peripheral blood （PB） was drawn from ear central artery of eight adult New Zealand white rabbits. BM-PRP and PB-PRP were obtained using two centrifugation techniques. The platelets in samples were counted manually and the data were statistically analyzed by t-test. Results The number of platelets increased 1.41-fold in samples of BM-PRP （2296.76 ±148.80） 103/ml compared to PB-PRP （ 1633.26 ± 89.64） 103/ml （ P〈 0.001 ）. Conclusion Bone marrow aspirate is one of an effective method to obtain BM-PRP , the number of platelets in BM-RPR samples was signifi- candy higher than PB-PRP samples.
出处
《中国美容整形外科杂志》
CAS
2013年第2期116-119,共4页
Chinese Journal of Aesthetic and Plastic Surgery
基金
国家重点基础研究发展计划（973）子课题资助项目（2009CB930003）
