摘要
建立了高效液相色谱法(HPLC)同时测定配方乳粉中7种脂溶性维生素A、D2、D3、E、K1、叶黄素和β-胡萝卜素的方法。方法采用Poroshell120SB-C18柱(150×4.6mm,2.7μm),以甲醇、乙腈、氯仿为流动相进行梯度洗脱,流速0.6mL/min,25min内可对上述7种组分进行基线分离。结果表明,7种维生素在各自相应浓度范围内线性关系良好,线性相关系数均大于0.999,平均加标回收率在83.0%～98.5%之间,相对标准偏差为0.84%～3.30%。该方法快速、准确、灵敏度高,适用于配方乳粉中脂溶性维生素含量的同时测定。
A method based on high performance liquid chromatography（HPLC） for the simultaneous determination of fat-soluble vitamin A, D2, Da, E, K1, Lutein and β-Carotene in infant formula was developed. HPLC analysis was performed on Poroshel1120 SB-C18 column（4.6 ×150 mm, 2.7 μm）using photo diode array（PDA） detection and gradient elution by methanol-acetonitrile-chloroform at 0.6 mL/ min in 25 minutes. The calibration curves of the seven fat-soluble vitamins were linear in respective concentration range with the correlation coefficients over 0. 999. Recoveries were from 83.0% to 98.5G and the RSDs were in the range of 0. 84 % - 3. 30%. This method was simple, sensitive, accurate and precise for batch determination of fat-soluble vitamins in infant formula.
出处
《分析科学学报》
CAS
CSCD
北大核心
2013年第1期109-112,共4页
Journal of Analytical Science
基金
浙江省质监系统科研计划(No.20120205)
