摘要
目的探讨用微波方法制备γ-干扰素(IFN-γ)处理的小鼠H22肝癌细胞瘤苗(MITTV-H22)的抗肿瘤效应。方法利用MITTV-H22免疫小鼠3次,末次免疫后第7天接种H22肿瘤细胞,观察肿瘤生长情况及检测小鼠淋巴细胞刺激指数。结果实验组小鼠于H22肿瘤细胞攻击后的7d接种部位没有发现肉眼可见的包块,经解剖肿瘤接种部位未发现肿瘤组织,120d后也未发现肿瘤组织。对照组于H22肿瘤细胞接种后第5或第6天接种部位出现直径小于0.5cm的包块,成瘤率为100%。观察120d,实验组小鼠平均生存期高于对照组(t=25.55,P<0.01)。脾淋巴细胞增殖实验检测结果显示,实验组淋巴细胞刺激指数高于对照组(t=3.526,P=0.01)。结论 MITTV-H22可以有效抑制肿瘤生长,具有明显的抗瘤效应。
Objective we made H22 tumor vaccine using IFN-γ and microwave treated H22 tumor cells（MITTV-H22） and explored the antitumor effects in the bodies immunized by tumor vaccine.Methods Mice were immunized with MITTV-H22 3 times and inoculated with H22 tumor cells at day 7 after last immunization.Tumor growth was observed and spleen cell proliferation was determined by MTT assay.Results In the experiment group,7 days after H22 tumor cells challenger,no visible tumors were found around the injection site,even if examined with anatomy.After 120 days,no tumors were found.In the control group,at day 5 or 6 after H22 tumor cells challenger,visible tumor（less than 0.5 cm in diameter） at the inoculation site was found with the tumor incidence of 100%.Observed 120 days,the average life span of experiment group was higher than that of control group（t=25.55,P0.01）.MTT assay showed that the stimulation index in experiment group was higher than that of control group（t=3.526,P=0.01）.Conclusion MITTV-H22 could effectively inhibit tumor growth and has antitumor immune response significantly.
出处
《检验医学与临床》
CAS
2013年第1期54-55,共2页
Laboratory Medicine and Clinic
