摘要
目的:建立通督活血丸的质量控制标准。方法:对处方中血竭、赤芍、片姜黄、秦艽进行薄层色谱定性鉴别,同时对赤芍中的芍药苷采用高效液相色谱法进行含量测定。结果:薄层鉴别专属性强,方法可行;芍药苷在2.41～8.44μg范围内呈良好的线性关系;回归方程为Y=2 044 088 088.823 1X-473 462.990 5,r=0.999 7;加样回收率平均值为99.51%,RSD为0.97%,加样回收良好。结论:所用方法简便、重现性好、专属性强,为通督活血丸质量评价提供了依据。
Objective：To study the quality standard of tongdu huoxue pill.Methods：To use thin layer chromatography to identify the xuejie,chishao,pianjianghuang and qinjiao.Use high performance liquid chromatography method for the determination of shaoyao content.Results：The thin layer chromatography is a feasible method with strong specificity.Shaoyaogan has a good linear relationship in in 2.41-8.44 μg.The regression equation for Y=2 044 088 088.823 1X-473 462.990 5,r=0.999 7.The coefficient of recovery was 99.51% and RSD was 0.97% with good sample recovery.Conclusion：The method is simple and reproducible with strong specificity to provide a basis for quality evaluation of tongdu huoxue pill.
出处
《中医学报》
CAS
2013年第1期78-81,共4页
Acta Chinese Medicine
基金
河南省科技创新人才计划(编号:114200510014)
关键词
通督活血丸
质量标准
高效液相色谱法
薄层色谱法
tongdu huoxue pill
quality standard
high performance liquid chromatography
layer chranatography
