摘要
为研究青岛大扁藻(Platymonas helgolandica var.Tsingtaoensis)和牟氏角毛藻(Chaetocerosmuelleri Lemmerman)的种间竞争关系,以KNO3和KH2PO4为N源和P源,各设5个质量浓度梯度,观察了2种微藻单种培养与共培养时的生长特性.结果表明:(1)单种培养时,青岛大扁藻密度与氮、磷质量浓度正相关(P<0.05);牟氏角毛藻密度与氮质量浓度正相关,但磷以1.14 mg.L-1组(P3)藻密度最高.(2)共培养时,2种藻的生长效果均不如单种培养,存在显著差异(P<0.05).(3)青岛大扁藻最适宜N/P比值为14.81,对磷有较高的需求;而牟氏角毛藻的最适宜N/P比值是26.93,其对较低的磷浓度具有更好的适应性.(4)共培养下,氮处理组青岛大扁藻占较大竞争优势,磷处理组牟氏角毛藻占更多竞争优势;在培养早期和末期青岛大扁藻具有明显的竞争优势,而在培养中期牟氏角毛藻竞争优势更大.
In order to study the interspecific competitive relationship of Platymonas helgolandica var. Tsingtaoensis and Chaetoceros muelleri Lemmerman, two kinds of microalgae＇s growth characteristics in single cultivation and cocultivation were observed using of KNO3 and KH2PO4 as N and P source at five mass concentration gradient. The results show that： （1） In single culture condition, P. helgolandica＇s density is positively related to nitrogen and phosphorus mass concentration （P〈0.05）, and C. muelleri＇s density is also positively related to nitrogen mass concentration. But algae density is found to be the highest in phosphorus 1.14 mg/L （P3）. （2） Growth effect of two kinds of algae in cocultivation condition is less than in single culture, which shows significant difference （P〈0.05）. （3） P. helgolandica＇s optimum N/P ratio is 14.81, which has higher demand for P. While C. muelleri＇s optimum N/P ratio is 26.93, which reveals the better adaptability to the low phosphorus concentration. （4） In cocultivation condition, the P helgolandica of nitrogen treatment group takes a significant competitive advantage. While the C. muelleri of phosphorus treatment group has more competitive advantages. P. helgolandica manifests the obvious competitive advantage in the early stage and late period of cultivation, and C. muelleri shows a greater competitive advantage in the middle period of cultivation.
出处
《宁波大学学报（理工版）》
CAS
2013年第1期12-17,共6页
Journal of Ningbo University：Natural Science and Engineering Edition
基金
国家海洋公益科研专项(201105009-3)
浙江省"新农村科技示范点建设"项目(XJKS2010001)
宁波市重大攻关项目(2011C11003)
关键词
青岛大扁藻
牟氏角毛藻
氮磷
共培养
种间竞争
Platymonas helgolandica var. Tsingtaoensis
Chaetoceros muelleri Lemmerman
nitrogen andphosphorus
cocultivation
interspecific competition
