摘要
为应用耐寒常春藤的有利性状,以嫩茎为材料,采用生根培养的方法,对其进行了生根培养、继代扩增培养以及移栽和移植的研究。结果表明:把无菌嫩茎的下部切口在浓度为10mg.L-1的IAA溶液中处理3min后,接种到1/2MS+蔗糖20g.L-1+NAA0.2mg.L-1～0.4mg.L-1的培养基上是耐寒常春藤无菌嫩茎生根培养的理想培养方法;把试管苗剪成具有侧芽或顶芽的茎段后,在1/2MS+蔗糖20g.L-1+NAA0.3mg.L-1的培养基上进行扩增培养是耐寒常春藤试管苗扩增培养的理想方法;试管苗移栽成活率在95%以上;定植2年后陆地越冬成活率在91%以上;试管苗保持了耐寒变异性状。
In order to preserve the wild resources of the cold--resistant Hedera nepalensis K,this article uses tender stems of Hedera nepalensis K as explants to do rooting cluture, and proliferates cultivation of roots and transplanting. The results indicate that the ideal medium for rooting of tender stems is 1/2 MS＋ sucrose （20goL^-1）＋NAA（0.2mg ·L^-1-0.4mg ·L^-1） with the method that the lower incision of sterile stems im merse at the 10mg ·L^-1 IAA solution in 3 minutes; after the seedlings are sheared into stems with lateral buds or terminal buds,the perfect medium for proliferating cultivation of them is 1/2MS＋ sucrose30 g/L ＋ NAA 0.3 mg/L; the transplanting survival rate is above 95 % ;after planting for two years the survival rate of the overwintering plants is above 91% and the test--tube plantlets maintain a cold--resistant characteristic.
出处
《绿色科技》
2013年第1期168-170,共3页
Journal of Green Science and Technology
基金
辽宁省普通高等教育本科教学改革研究项目(编号:201203041-4)
辽宁省大学生创新创业训练项目(编号:201203015011)资助
关键词
常春藤
耐寒
试管苗
Hedera nepalensis K
cold tolerance
test-- tube plantlet
