摘要
目的探讨羊睾丸提取液对铅染毒小鼠睾丸氧化损伤的拮抗作用。方法将30只健康清洁级ICR雄性小鼠随机分为对照(蒸馏水)组、模型组和羊睾丸拮抗组。模型组采用0.4%乙酸铅溶液灌胃染毒(染毒剂量为30 mg/kg,染毒容量为10 ml/kg),同时,腹腔注射蒸馏水0.5 ml;羊睾丸拮抗组采用0.4%乙酸铅灌胃染毒,同时,腹腔注射羊睾丸提取液0.5 ml。每天染毒1次,连续21 d。检测血清中睾酮(T)、卵泡刺激素(FSH)及黄体生成素(LH)含量;检测睾丸组织匀浆中总抗氧化能力(T-AOC)、总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)含量;检测附睾精子密度、精子活率和活精子百分率。结果与模型组比较,对照组和羊睾丸拮抗组小鼠血清T含量和睾丸组织匀浆中T-AOC、T-SOD、CAT、GSH-Px活力及精子密度、精子活率、活精子百分率均较高,MDA含量均较低,差异有统计学意义(P<0.01);而羊睾丸拮抗组小鼠血清T含量和睾丸组织匀浆中T-AOC、T-SOD、CAT、GSH-Px活力及精子密度、精子活率、活精子百分率与对照组比较,差异无统计学意义。各组小鼠血清FSH和LH含量间比较,差异均无统计学意义。结论羊睾丸提取液可拮抗铅染毒致小鼠睾丸的氧化损伤作用。
Objective To investigate the antagonism of caprine testicular extract against lead-induced testicular oxidative damage in mice. Methods The study was carried out on 30 adult male ICR mice divided randomly into control（distilled water） group,model group and antagonistic group. Model group was brought out by intragastric administration of 0.4% lead acetate（30 mg/kg body weight at does, 10 ml/kg at capacity） and intraperitoneal injection of distilled water（0.5 rnl） at the same time. The antogonistie group received intragastric administration of 0.4% lead acetate and intraperitoneal injection of caprine testicular extract（0.5 ml） for 21 days. The effects of these treatments were detected on the levels of plasma T, FSH and LH,the activities of testicular total antioxidant capacity （T-AOC）,total superoxide dismutase （T-SOD）,catalase（CAT）,glutathione peroxidase （GSH-Px） and the content of testicular malondialdehyde （MDA）. Sperm density,sperm motility and percentage of live sperm in epididymis were detected as well. Results Compared with the model group,the level of plasma T,the activities of testicular T-AOC,T-SOD,CAT and GSH-Px,epididymal sperm density,sperm motility and percentage of live sperm were all increased,and the content of MDA decreased in the control group and the antogonistic group with significant differences （P〈 0.01 ）. Compared with the control group,all of the above indicators in the antogonistic group had no significant differences. The levels of plasma FSH and LH between any two groups had no significant difference. Conclusion The caprine testicular extraet can antagonize lead-induced oxidative damage in testis of mice.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2013年第1期24-26,共3页
Journal of Environment and Health
基金
吉林省教育厅科学技术研究项目(2012485
2012484)
关键词
羊睾丸提取液
铅
小鼠
氧化损伤
Caprine testicular extract
Lead
Mice
Oxidative damage
