摘要
本实验采用门静脉缩窄法建立门静脉高压巨脾动物模型,行巨脾大都切除,一组行带血管蒂部分巨脾髂部移植,一组行单纯部分巨脾原位保留。术后不同时间内比较两组保留脾在大体形态、免疫功能上有何不同;并根据形态测量学方法,作脾组织定量分析,比较两组脾显微结构的差异。结果证明体外吞噬试验移植脾优于原位保留脾(P<0.01)。组织学发现原位保留脾术后两月的脾被膜厚度,脾小梁宽度测定值均大于移植脾(P<0.01),脾小体直径小于移植脾(P<0.05)。尤其是原位保留脾术后一年的纤维化程度与术后1月、2月相比呈进行性加重(P>0.01)。实验并对巨脾骼部移植的方法及可行性进行了详细论述。
In our study, portal hypertension was produced in experimental animals by the stenosis of portal vein and then, partial resection of splenomegaly was performed. The partial splenomegaly with vessel pedicle was transplanted to the illiac site in one group and in the other group, the partial splenomegaly was preserved in situ. At different times after operation, a compariton between the two group was made of the morphologic changes as well as the function of splenomegaly. The comparison of their microstructure was also instituted by histological quantitative analysis according to morphometric method. The results showed that the splenic function in the preservation-in-situ group was not better than that in the transplanted group by the test of phagocytosis in vitro ( P<0.01 ) . Histology showed that two months after operation, the thickness of splenic capsule and the width of splenic trabecule were greater in the preservation group ascompared with those in the transplantation group (P<0.01), and the diameter of splenic nodule was less than later ( P<0.01 ). Especially the fibrosis of the preserved splenomegaly in situ one year after operation, compared with themselves one and two months after operation, was progressively accelerated with time ( P<0.0l ) . The technique of transplantation and operation feasiblity also be discussed in the study.
出处
《蚌埠医学院学报》
CAS
1991年第1期55-59,共5页
Journal of Bengbu Medical College
关键词
门脉高压
巨脾
移植
保脾
定量分析
portal hypertension
splenomegaly
transplantation
quantitative analysis
morphmetricology
