摘要
目的 :研究穿心莲是否具有体外破坏内毒素的作用。方法 :1电镜观察经穿心莲作用后的脂多糖(L PS)构型变化。 2观察穿心莲体外破坏 L PS的量效关系。 3观察穿心莲对 L PS刺激体外腹腔巨噬细胞分泌炎性细胞因子活性的影响。结果 :电镜观察证实经穿心莲作用后的 L PS已失去其正常结构 ,呈散乱分布的残破网或短带状 ,而且穿心莲体外破坏 L PS作用在一定范围内随药物浓度的增加而提高。 1g穿心莲约可裂解836 EU的 L PS。同时 ,穿心莲能轻度增加体外正常腹腔巨噬细胞分泌肿瘤坏死因子 α(TNFα)、白介素 1(IL 1)和 IL 6的活性 ,显著抑制经 L PS刺激体外腹腔巨噬细胞分泌大量上述炎性细胞因子的活性 ,其作用在一定范围内呈剂量依赖性。结论 :穿心莲在体外可通过直接破坏内毒素结构和抑制其所介导释放的大量炎性细胞因子活性起到拮抗内毒素的作用。
Objective:To research the destructive action of andrographis paniculata nees(APN) on endotoxin in vitro.Methods:The structural changes of lipopolysaccharide(LPS) acted by APN,the relationship of the destructive action of APN on LPS between volume and efficacy in vitro and the influence of APN on activitie of inflammatory cytokines secreted by macrophages of peritoneal cavity stimulated by LPS in vitro were observed.Results:By means of electromicroscopic observation it was showed that LPS lost its normal structure and became scattered and destroyed netshape or shortbelt shape after the action of APN.The action of APN on destroying LPS in vitro was increased in certain extent as the concentrations were heightened.1 g of APN could cleavage about 836 EU LPS.Meanwhile,APN could slightly increase the activities of inflammatory cytokines 〔tumor necrosis factorα(TNFα),interleukin1(IL1) and IL6〕 secreted by the normal macrophages of peritoneal cavity,whereas it could significantly inhibit the activities of inflammtory cytokines secreted by the macrophages of peritoneal cavity stimulated by LPS.Furthermore,these action of APN were dosedependent.Conclusions:APN has the substantial action on destroying endotoxin in vitro through destroying the structure of LPS directly and inhibiting the activities of inflammtory cytokines induced by LPS.
出处
《中国中西医结合急救杂志》
CAS
2000年第4期212-214,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
天津市科委科研基金
关键词
穿心莲
内毒素
超微结构
巨噬细胞
中药
andrographis paniculata nees
endotoxin
ultrastructure
macrophage
cytokine
traditional Chinese medicine
