摘要
利用TN5插入强致病力青枯雷尔氏菌FJAT-91,获得突变株FJAT-t582和FJAT-t583。菌落形态观察及弱化指数测定结果表明,突变株FJAT-t582和FJAT-t583表现为典型的无致病力菌落形态,且弱化指数均在0.8以上。利用卡那霉素抗性基因特异性引物从突变菌株FJAT-t582和FJAT-t583中均扩增出片段大小为681bp的条带,而野生型菌株FJAT-91未扩增出任何条带,说明TN5转座子已插入其基因组中。对这2株无致病力突变株进行反向PCR测序和序列比对,分析鉴定出插入位点在编码putative diguanylate phosphodiesterase基因和Ⅲ型效应蛋白基因中,推测这2个基因的突变致使强致病力菌株表现出无致病力特征。
Mutants of Ralstonia solanacearum strain FJAT-91 using Tn5 transposon mutagenesis were developed in this paper,which were named FJAT-t582 and FJAT-t583.Colony morphology and attenuation indexes showed that both of the mutants FJAT-t582 and FJAT-t583 were avirulent and their attenuation indexes were over 0.8.A 681 bp fragment of the kanamycin resistance gene was amplified from the two genome DNA of mutants.The insertion sites were located in putative diguanylate phosphodiesterase and type Ⅲ effector protein gene respectively.And they may cause the pathogenicity reduction of the R.solanacearum.
出处
《福建农业学报》
CAS
2012年第11期1231-1236,共6页
Fujian Journal of Agricultural Sciences
基金
国家"973"计划前期项目(2011CB111607)
国家"948"计划项目(2011-G25)
国家自然科学基金项目(30871667)
"十二五"农村领域国家科技计划课题(2012AA101504)
国家星火科技计划项目(S20110410006)
福建省财政专项--福建省农业科学院科技创新团队建设项目(STIF-Y03)
