摘要
为对1株鸽源鸭甲肝病毒1型(DHAV-1)进行分离与鉴定,通过细菌分离排除法和病毒分离方法获得致鸭胚死亡病毒(暂命名为FJ-12-20株)。该病毒无血凝活性,可被DHAV-1引物所扩增,但不能被鸭坦布苏病毒、番鸭呼肠孤病毒、番鸭细小病毒和鹅细小病毒特异引物所扩增,病毒液攻击樱桃谷鸭和番鸭可引起死亡,并出现了典型的鸭肝炎病变,重新分离获得病毒株。将DHAV-1特异性扩增产物回收后克隆,序列分析表明FJ-12-20株与参考株DHAV-1的同源率为93.5%～99%,与DHAV-2和DHAV-3参考株的同源率均为78.4%。遗传进化分析表明FJ-12-20株与DHAV-1关系密切,它们在进化树中共处一分支。
Duck hepatitis A virus 1 （DHAV-1）, FJ-12-20, was isolated from a dead pigeon in Fujian. The strain, which lacked the hemagglutination activity, was identified by using the specific primers of DHAV-1, against those of the duck Tembusu virus, duck reovirus, duck parvirus and goose parvirus. Some Cherry Valley ducks and Muscovy ducks died after being challenged by the allantoic fluid containing FJ-12-20. Those ducks characterized with overt lesions of duck hepatitis. The virus was also successfully recovered from the infected ducks, and confirmed by reverse transcription-PCR. Sequence analysis revealed a high homology （93.5%- 99 %） existed between FJ-12-20 and DHAV-1 from GenBank. A significantly lower homology （78.4%）, on the other hand, was found between FJ- 12-20 and the standard strain of DHAV-2 or DHAV-3. Phylogenetic analysis showed that FJ-12-20 was close to DHAV-1, and all of these viruses could be subdivided into an isolated cluster.
出处
《福建农业学报》
CAS
2012年第11期1151-1154,共4页
Fujian Journal of Agricultural Sciences
基金
福建省自然科学基金项目(2011J01113)
国家自然科学基金项目(30471283)
福建省种业创新与产业化工程建设项目(2011FJZY-9-06)
关键词
鸽源
鸭甲肝炎病毒1型
分离鉴定
pigeon-origin
duck hepatitis A virus 1
isolation and identification
