摘要
目的用改良的沙门菌分离鉴定方法提高肠道沙门菌的检出率,初步掌握上海市主要沙门菌流行状况及菌型分布,建立沙门菌脉冲场凝胶电泳(PFGE)分型数据库,以便在发生沙门菌病时快速溯源,为控制该病的传播提供技术支撑。方法用改良的沙门菌鉴定方法对可疑肠道粪便标本进行分离、培养和鉴定;用PFGE对鼠伤寒沙门菌进行DNA分子分型,分析试验菌株的同源性。结果用改良的沙门菌鉴定方法分离出的沙门菌中以肠炎沙门菌为主,占全部菌株的42.1%,其次为鼠伤寒沙门菌,占13.2%。PFGE分析的10株鼠伤寒沙门菌分成A、B 2个PFGE型,其中A型菌株6株,B型菌株4株。A型菌株有4个亚型。结论改良的沙门菌鉴定方法可以有效提高沙门菌的检出率;PFGE是一种研究鼠伤寒沙门菌分子分型的有效方法,鼠伤寒沙门菌的分型结果显示了相对集中的亲缘关系。
Objective To increase the detection rate of Salmonella in intestinal tract by the improved isolation and identification method,and to fundamentally understand the major Salmonella and its molecular typing in Shanghai,establish the database of pulsed-field gel electrophoresis(PFGE)typing for rapid source-tracing and control dissemination of the disease during Salmonella outbreaks.Methods The improved isolation and identification method was used to detect suspected Salmonella in fecal specimens.PFGE was used to perform molecular typing of Salmonella typhimurium.Results Salmonella enteritidis was main among the isolates of Salmonella by the improved isolation and identification method,accounting for 42.1%.The second was Salmonella typhimurium,accounting for 13.2%.Two distinct DNA fragment profiles,PFGE type A and B,were observed,including type A: 6 isolates and type B: 4 isolates respectively.Type A could be subclassified further into 4 subtypes.Conclusions The improved isolation and identification method could effectively increase the detection rate of Salmonella in fecal specimens.PFGE is an effective method to study the molecular typing.The research shows relatively close kinship in molecular typing of Salmonella typhimurium.
出处
《检验医学》
CAS
2012年第11期913-916,共4页
Laboratory Medicine
关键词
沙门菌
改良分离鉴定方法
脉冲场凝胶电泳
分子分型
Salmonella
Improved isolation and identification method
Pulsed-field gel electrophoresis
Molecular typing
