摘要
目的评价16S～23S rRNA基因转录间隔区聚合酶链反应(ITS-PCR)在非结核分枝杆菌(NTM)菌种鉴定中的临床价值。方法采用ITS-PCR鉴定随机抽取的80株NTM,应用生长特性鉴定试验及鉴定性药物敏感性试验鉴定其表型特征,将3种鉴定方法所示结果进行对比分析。结果 80株NTM中,鸟-胞分枝杆菌复合群(MAC)与脓肿分枝杆菌共占85%,运用ITS-PCR可将脓肿分枝杆菌、堪萨分枝杆菌、偶发分枝杆菌、苏尔加分枝杆菌准确鉴定,与生长特性鉴定试验及鉴定性药物敏感性试验结果基本符合;MAC采用3种方法鉴定其结果符合率存在偏差。结论 NTM ITS-PCR鉴定法与表型鉴定法相结合更适合临床应用。
Objective To evaluate the clinical significance of 16S-23S rRNA intergenic transcribed spacer-polymerase chain reaction（ITS-PCR）identification for nontuberculous Mycobacterium（NTM）.Methods A total of 80 NTM identified by ITS-PCR were randomly selected.Phenotypic characteristics of NTM were identified by growth characterization test and drug sensitivity test.The results for the 3 identification methods were analyzed contrastively.Results The proportion of Mycobacterium avium complex（MAC） and Mycobacterium abscessus was 85%.Mycobacterium abscessus,Mycobacterium kansasii,Mycobacterium fortuitum and Mycobacterium szulgai were identified accurately by ITS-PCR.The results were consistent among the results of growth characterization test,drug sensitivity test and ITS-PCR except that of MAC.Conclusions The combination of ITS-PCR with the phenotypic identification for NTM is suitable to the clinical application.
出处
《检验医学》
CAS
2012年第11期908-912,共5页
Laboratory Medicine
关键词
非结核分枝杆菌
基因转录间隔区聚合酶链反应
生长特性鉴定试验
药物敏感性试验
Nontuberculous Mycobacterium
Intergenic transcribed spacer-polymerase chain reaction
Growth characterization test
Drug sensitivity test
