摘要
目的 了解术后胃癌的多药耐药性与胃癌组织中P gp过量表达的关系。 方法 利用免疫组化方法 ,以 4种抗不同亚型P gp(C494,MRK16 ,JSB 1和C2 19)的单克隆抗体 (mAb) ,以及 4种不同抗P gpmAb的混合抗体和抗p5 3蛋白mAb ,分别检测 6 6例胃癌术后石蜡包埋组织中P gp和p5 3的表达 (其中 16例为冰冻切片 )。 结果 P gp呈中等或高度表达 (占 40 %～ 70 % )。依次为 :C494( 75 75 % ,5 0 /6 6 ) ,JSB 1( 6 2 12 % ,41/ 6 6 ) ,MRK16 ( 48 49% ,32 / 6 6 )和C2 19( 36 36 % ,2 4/ 6 6 )。 4种抗P gpmAb的混合物的阳性检出率为 78 79% ( 5 2 / 6 6 ) ,抗p5 3蛋白mAb的阳性检出率为6 0 6 1% ( 40 / 6 6 )。 4种抗P gpmAb的混合物与抗P gpmAbC494(G =0 90 ,P <0 0 0 0 0 1) ;抗P gpmAbC494与抗JSB 1mAb(G =0 6 0 ,P <0 0 0 0 0 5 ) ;抗MRK16mAb与抗C2 19mAb(G =0 6 4,P <0 0 0 0 1)均高度相关。石蜡和冰冻切片中P gpC494和JSB 1呈中度表达以上者阳性率基本一致 ;而C2 19和MRK16的阳性率相差则明显 (P <0 0 5 )。p5 3蛋白的表达与C494和JSB 1的表达相关性显著 (P <0 0 0 2和P <0 0 0 2 ) ,与MRK16和C2 19的表达也有相关性(P <0 0 1和P <0 0 5 )。结论 C494阳性表达率最高 ,可作为胃癌组织中耐药基?
Aim To understand relationship between multidrug resistance of postoperative gastric cancer and Pgp overexpression in the gastric cancer tissues. Methods Paraffin sections from 66 cases of gastric carcinoma(16 cases of them were frozen sections) were immunohistochemically(IHC) analyzed using a panel of 4 different antipglycoprotein monoclonal antibodies (mAbs) composed of C494, MRK16, JSB1 and C219. Cocktail of the 4 mAbs and mAb against p53 protein were also used. Results Moderate to high levels of pglycoprotein were detected in 40% to 70% of gastric cancer. The positive rate of C494 (7575% , 50/66)was higher than those of JSB 1(62.12% ; 41/66), MRK16(48.49% , 32/66)and C219(36.36% , 24/66). The positive rate of cocktail of the 4 antipglycoprotein antibodies was 78.79% (52/66). The positive rate of p53 protein was 60.61% (40/66). The positive expression of cocktail of the 4 antipglycoproteins was closely related to 4 antipglycoprotein C494(G=090, P<000001); The positive expression of C494 was closely related to JSB1(G=060,P<000005). The positive expression of MRK16 was closely related to C219(G=064, P<00001). There were similar positive expressions of C494 and JSB1 between corresponding paraffin and frozen sections, but different ones between C219 and MRK16. Expression of p53 protein was closely related to C494(P<0002), JSB1 (P<0002), MRK16 (P<001) and C219 mAbs(P<005). Conclusion The positive expression of C494 was the highest and could be as a marker for multidrug resistance gene assay. Overexpression of pglycoprotein was closely related to p53 protein.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
2000年第3期264-267,共4页
Chinese Journal of Cellular and Molecular Immunology
