摘要
目的回顾性分析该院2010年1月至2011年12月儿童血培养病原菌分布和耐药性特点,为该院儿童血流感染抗菌药物使用提供依据。方法血培养采用BACTEC自动化血培养仪,菌株鉴定使用VITEK-32细菌鉴定系统或API鉴定系统。结果 2134例血培养标本中共分离210株细菌,其中革兰阳性菌株157株(占74.76%),革兰阴性菌株53株(占25.24%)。耐甲氧西林金黄色葡萄球菌占金黄色葡萄球菌56.12%,耐甲氧西林凝固酶阴性葡萄球菌占凝固酶阴性葡萄球菌79.07%,未检出耐万古霉素、替考拉宁革兰阳性球菌;大肠埃希菌和肺炎克雷伯菌产超广谱β-内酰胺酶分别占55.56%、46.15%,未检出耐碳青霉烯革兰阴性细菌。结论该院儿童血培养主要病原菌为革兰阳性菌,且耐药株比例较高,重视抗菌药物的合理使用刻不容缓。
Objective To investigate the distribution of pathogenic bacteria isolated from the children＇s blood culture and to analyze the antibiotic resistance in the last two years to provided basis for the usage of antimicrobial agents.Methods Blood samples were cultivated by BACTEC automated system and pathogenic bacteria were identified by VITEK-32 or API system.Results 210 pathogens could be isolated from 2 134 blood culture specimens,among which 157 strains（74.76%） were Gram-positive bacteria and 53 strains（25.24%） were Gram-negative bacteria.Methicillin-resistant Staphylococcus aureus accounted for 56.12% and methicillin-resistant coagulase negative Staphylococcus accounted for 79.07%.Gram-positive bacteria were sensitive to vancomycin and teicoplanin.The extended spectrum beta-lactamases of E.coli and K.pneumonia accounted for 55.56% and 46.15%.Gram-negative bacteria were sensitive to carbapenem.Conclusion Gram-positive bacteria might be the predominant pathogens in children＇s patients and resistant against various antibiotics.The rational usage of antimicrobial drugs might be very important.
出处
《国际检验医学杂志》
CAS
2012年第15期1840-1842,共3页
International Journal of Laboratory Medicine
关键词
葡萄球菌
金黄色
血流感染
细菌
培养的
抗药性
细菌
Staphylococcus aureus
blood stream infection
cells
cultured
drug resistance
bacterial
