摘要
SmartAmp是一种新的DNA等温扩增技术,具有操作简单、快速、成本低、灵敏度高、特异性强、背景低等优点。目前已在临床基因多态性检测、感染性疾病诊断等方面初步应用,为临床快速诊断提供了帮助,在食品和环境中病原体检测领域也显示了极大的应用发展潜力。我们简要概述SmartAmp技术原理、特点及其应用前景。
SmartAmp, the Smart Amplification Process, is a novel isothermal DNA amplification technology. It has advantages like simplicity, rapidity, cost effectiveness and background suppression, and also displays high sensi- tivity and strong specificity. The technology has been preliminarily applied in clinical genetic polymorphisms detec- tion and infectious disease diagnostics. It promotes the development of clinical rapid diagnostic and shows huge po- tential in the detection of environmental or food contaminants. In this article, we reviewed the mechanism and fea- tures of SmartAmp and prospected its amplification.
出处
《生物技术通讯》
CAS
2013年第1期139-142,共4页
Letters in Biotechnology
基金
国家科技重大专项(2008ZX10004-008
2009ZX10004-315)
国家自然科学基金(81000723)
