摘要
α毒素是产气荚膜梭菌主要的毒力因子,具有磷脂酶C和鞘磷脂酶等2种酶的活性,能同时水解细胞膜上的磷脂酰胆碱和鞘磷脂,导致细胞裂解,因而具有细胞毒性、溶血活性、致死性和血小板聚集等特性。我们对产气荚膜梭菌α毒素的生物学特性、基因克隆与转录调控、基因表达与基因融合、基因定点突变与结构分析、N端和C端的结构与功能等方面的研究进展进行简要综述。
Alpha-toxin is a major pathogenic determinant of Clostridium perfringens and exhities both phospholi- pase C and sphingomyelinase activities. It can hydrolysis phosphatidylcholine and sphingomyelin. It causes cell ly- sis and possesses cell toxicity, hemolysis, lethality, and platelet aggregation. In this paper we summarized the re- searches on biological characteristics, gene cloning and transcriptional regulation, gene expression and gene fusion, site-directed mutagenesis and structural analysis, structure and function of N- and C-domain of C.perfringens al- pha-toxin.
出处
《生物技术通讯》
CAS
2013年第1期113-117,共5页
Letters in Biotechnology
基金
国家自然科学基金(30972188)
关键词
产气荚膜梭菌α毒素
转录调控
基因表达
定点突变
结构与功能
Clostridium perfringens alpha-toxin
transcriptional regulation
gene expression
site-directed mutagen- esis
structure and function
