摘要
目的:观察截断逆挽方对慢加急性肝衰竭大鼠血清TNF-α、IL-1β及IL-6的影响。方法:将61只wistar大鼠分为正常组、模型组及中药组。采用猪血清免疫诱导大鼠肝纤维化或肝硬化模型,再给予D-GalN/LPS一次性联合腹腔注射进行急性攻击,建立慢加急性肝衰竭大鼠模型。中药组在急性攻击前给予截断逆挽方水煎剂连续灌胃3 d;模型组及中药组在急性攻击后4、8、12 h分批麻醉处死大鼠。观察大鼠血清TNF-α、IL-1β、IL-6、ALT及AST含量变化;HE染色观察各组大鼠肝脏病理变化。结果:与模型组同时相点比较,中药组能显著降低模型大鼠血清ALT、AST、TNF-α、IL-1β及IL-6的含量,减轻肝组织的病理损伤。结论:截断逆挽方可降低慢加急性肝衰竭大鼠血清TNF-α、IL-1β及IL-6等炎性细胞因子的含量,减轻慢加急性肝衰竭时肝组织的损伤。
Objective：To explore IL-6 of serum in Acute-on-chronic the Effects of the Prescription of Truncation Liver Failure Model Rats. Methods： The 61 and Inverse Draft on the TNF-α、IL-1β and wistar rats were divided into normal group, model group and Chinese medicine group. The pig serum was used to induce immunly rat liver cirrhosis or liver fibrosis model, and then D-GalN/LPS was given by intraperitoneal injection to establish Acute-on-chronic Liver Failure Model. Before acute attacked, the Chinese medicine group was feed liquid of the Prescription of＇Truncation and Inverse Draft＇three days. Af- ter acute attacked 4, 8 and 12 h, Model group and Chinese medicine group were respectively put to death number rats. the changes of seruml indexes ALT and AST of every group rats were observed; Liver pathology changes situation in each group rat was observed by HE staining method;The content of TNF a, IL-1 [3 and IL-6 in rat serum were observed by ELISA method. Results： Compared to the corresponding time points model group, The Chinese medicine groupcan observably reduce the content of ALT, AST, TNF-α、IL-1β and IL-6 in rat serum, alleviate the liver tissue pathological damage. Conclusions： The Prescription of ＂Truncation and Inverse Draft＂ can decrease TNF-α、IL-1β、IL-6 etal cells factor content to lighten liver tissue damage in acute-on-chronic liver failure.
出处
《中医药导报》
2013年第1期8-11,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
北京市自然基金项目(7112017)
