摘要
为了建立黑武士大叶醉鱼草的快速繁殖与遗传育种体系,以大叶醉鱼草的茎段为外植体,研究其离体培养及试管苗再生途径。结果表明:愈伤组织诱导的最佳培养基为MS+2.0mg/L 2,4-D+0.2mg/L 6-BA,诱导率为85%;愈伤组织分化的最佳培养基为MS+2.0mg/L TDZ+0.2mg/L NAA,分化率为80%,平均单芽数高于13个;最佳生根培养基为1/2MS+0.5mg/L IBA,生根率为78.7%,移栽成活率为80%。
The isolated culture and tube seedling regeneration of B. davidii explants were studied to establish a rapid propagation and genetic breeding system of Heiwushi B. davidii. The results showed that the optimum culture media for calli induction and differentiation were MS＋ 2. 0 mg/L 2,4-D＋0. 2 mg/L 6-BA and MS＋2.0 mg/L TDZ＋0.2 mg/L NAA with the induction rate and differentiation rate of 85% and 80% separately. The average single bud number was 13. The optimum rooting medium was 1/2 MS＋0.5 mg/L IBA. The rooting rate and survival rate were 78.7% and 80% respectively.
出处
《贵州农业科学》
CAS
北大核心
2013年第1期31-33,共3页
Guizhou Agricultural Sciences
基金
国家转基因新品种培育重大专项子项目"转基因生物的基因删除和基因拆分技术研究"(2011ZX08010-003)
贵州省高技术产业化示范工程项目"贵州大学贵州省植物分子育种工程研究中心创新能力建设"[黔发改高技(2010)2400]
贵州省植物基因工程科技创新人才团队项目"贵州省植物基因工程科技创新人才团队"[黔科合人才团队(2011)4001]
