摘要
为保护与发展棘胸蛙资源,利用RAPD技术对贵州省宽阔水国家级自然保护区一个棘胸蛙种群的20只标本进行遗传多样性检测。结果表明:用5条引物检测出37个RAPD位点,其中,多态位点31个,多态位点百分率83.78%。种群内Nei基因多样性指数为0.324 6±0.190 5,Shannon多样性指数为0.473 5±0.259 3。最大遗传距离为0.871,最小遗传距离为0.095,平均遗传距离为0.296,平均遗传相似系数为0.704。表明,宽阔水国家级自然保护区该种群的棘胸蛙具有较高的遗传多样性。
For the sake of providing theoretical support for protection and reasonable development of P. spinosa resources, DNA polymorphism of 20 individuals of P. spinosa in Kuankuoshui National Natural Reserve was analyzed by random amplified polymorphic DNA （RAPD）. Results： A total of 37 loci, including 31 polymorphic ones, were detected from the 20 individuals by using five primers. The average percentage of polymorphic loci to total DNA of the 20 individuals was 83.78%. The population had on average the Nei＇s value of 0. 324 6±0. 190 5 for gene diversity, and the Shannoffs information index of 0. 4735±0. 2593. The maximum genetic distance was 0. 871, the minimum genetic distance was 0. 095, the average genetic distance was 0. 296, and the average genetic similarity was 0. 704. There existed a relatively abundant genetic diversity in the population.
出处
《贵州农业科学》
CAS
北大核心
2013年第1期14-17,共4页
Guizhou Agricultural Sciences
基金
合肥市重大科技项目培育与科技人才培养计划项目"宽阔水国家级自然保护区无尾两栖类资源及遗传多样性的研究"[遵科培字(2008)04]
关键词
宽阔水自然保护区
棘胸蛙
遗传多样性
Kuankuoshui National Natural Reserve
Paa spinosa
genetic diversity
