摘要
目的:观察从肺论治法和从肠论治法对克罗恩病(CD)大鼠转化生长因子-β1(TGF-β1)含量的影响。方法:采用三硝基苯磺酸(TNBS)水溶液+无水乙醇一次灌肠法建立大鼠克罗恩病模型后分别灌喂从肺论治方(黄芪桔梗汤)、从肠论治方(黄芪黄连汤)和西药柳氮磺胺吡啶(SASP)进行实验性治疗,然后于0周、第3周两个时间点测定肺和结肠TGF-β1蛋白表达、TGF-β1 mRNA转录水平变化及血清中TGF-β1水平。结果:模型组大鼠0周时,结肠及肺组织中TGF-β1蛋白和TGF-β1 mRNA转录水平均较正常组显著升高(P<0.01),血清TGF-β1水平显著性高于正常组(P<0.01);3周后,模型组仍较正常组显著性性升高,各治疗组均较模型组显著性下调(P<0.01,P<0.05)。在下调结肠组织TGF-β1蛋白水平上,西药组虽然较模型组降低(P<0.05),但与正常组之间亦存在差异(P<0.01);且效果低于与中药从肠论治组(P<0.05)。而中药从肺论治组和从肠论治组与正常组之间没有明显差异。在下调肺组织TGF-β1 mRNA转录水平上,西药组与从肠论治组虽然较模型组降低(P<0.01),但与正常组之间亦存在差异(P<0.01)。而从肺论治组与正常组之间没有明显差异,但与从肠论治与西药组间存在差异(P<0.05)。在下调血清TGF-β1水平上,各治疗组虽较模型组下降但与正常组亦存在差异(P<0.01,P<0.05),而且从肠论治组较从肺论治组显著性降低(P<0.01)。结论:中西药物均能下调CD肺、肠组织、及血清中过度表达的TGF-β1,起到抗炎、促进修复、预防纤维化的作用;从肠论治方在减轻CD结肠组织的炎症反应、预防和减轻结肠组织纤维化方面效果突出;从肺论治方减轻肺内炎症和损伤,防止肺纤维化形成方面效果突出。
Objective： To observe the effects of TCM from the lung therapy and from the intestine therapy on the level of TGF-~31 in rats with Crohn＇s disease. Methods： The rat model of Crohn＇s disease was established by TNBS and ethanol enema. The model rats were experimentally treated with from the lung therapy decoction （Huangqijiegeng Decoction）, from the intestine therapy decoction （Huangqihuanglian Decoction）, and western medicine SASP, respectively, by gastric perfusion. And the changes in the level of TGF-β1, TGF-β1 mRNA in colon and lung, the level of TGF-β1 in blood serum of rats with crohn＇s disease were respectively detected at the period of time of zero and three weeks after the treatment. Results： In the zero week,the level of TGF-β1 and TGB-β1 mRNA in colon and lung in model group, the level of TGB-β1 in blood serum was significantly increased compared with that in normal control group（P〈0.O1）. Three week after treatment, model group was still significantly increased compared with that in normal control group（P〈0.01）, and in different treating group the level of TGF-β1 was significantly decreased compared with that in model group（P〈0.01,/~〈0.05）. The level of TGF-β1 of intestine in SASP group was decreased than model group（P〈0.05）, but there was obvious differences between SASP group and normal control group either（P〈0.01）; and lower than the level of from-intestine therapy group（P〈0.05）. There was no obvious difference between from the intestine therapy group and from the lung therapy group compared with normal control group. The level of TGB-β1 mRNA of lung in SASP group and from the intestine therapy group was decreased compared with that in model group（P〈0.01）, but there was obvious difference between them and normal control group（P〈0.01）. There was no obvious difference between from the lung therapy group and normal control group, but there was obvious differences between the SASP groupand form the intestine group（P〈0.05）. The level of TGF-β1 in blood serum was decreased in different treating groups compared with model group, but there was obvious difference with normal control group（P〈0.01, P〈0.05）, from the intestine therapy group decreased significantly than from the lung therapy group（P〈0.01）. Conclusion： All of the three kinds of medicine including from-lung therapy decoction （Huangqijiegeng Decoction）, from-intestine therapy decoction （Huangqihuanglian Decoction） and sulfasalazine could decrease the expression of TGF-β1 in rats with crohn＇s disease. From the intestine therapy decoction had an advantage in relieving the inflammatory reaction and preventing intestinal fibrosis. From the lung therapy decoction had an advantage in relieving inflammatory reaction in lung and preventing pulmonary fibrosis.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2013年第2期371-378,共8页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家重点基础研究发展计划(973计划)(No.2009CB522705)~~
