摘要
目的:探讨多烯磷脂酰胆碱对大鼠自体肝移植术后肝细胞增殖的影响。方法:采用门静脉灌注的大鼠自体肝移植模型:A组为多烯磷脂酰胆碱组。B组为单纯大鼠自体肝移植组。C组为正常对照组。比较大鼠自体肝移植术后肝脏组织肝细胞线粒体损伤变化和肝细胞PCNA变化。结果:术后12 h,多烯磷脂酰胆碱组大鼠自体肝移植术后肝细胞内线粒体肿胀较轻,椭圆形,内嵴部分断裂,但仍有少量排列。周围内质网结构尚存。肝细胞PCNA阳性细胞比率为0.87±0.24(P<0.05);较大鼠单纯自体肝移植组(0.75±0.32)增殖较多。单纯自体肝移植组肝细胞内线粒体大小不一,明显肿胀,呈类圆形,内有空泡变性,严重者可见嵴减少、断裂或消失;对照组大鼠肝细胞线粒体无明显损伤,形态可,无异常。术后12 h肝细胞PCNA阳性细胞比率为0.39±0.16(P<0.05)。结论:多烯磷脂酰胆碱可保护肝细胞,通过保护线粒体及促进肝细胞增殖,减少肝功能损伤。对大鼠肝移植术后肝脏恢复起促进作用。
Objective： To investigate the Effect of Polyene phosphatidylcholine for Liver cell proliferation after the rat liver autologous transplantation. Methods： Group A： the polyene phosphatidyl choline group. Group B： the liver autologous transplantation group. Group C： the control group. Comparison of three groups＇ changes with liver mitochondrial damage and liver cells prolifer- ation ~PCNA）. Results.. After 12h, the mitochondria from group A is a little swelling, oval, inner ridge part of the fracture, but most is still normal. PCNA-positive cells in the liver cells ratio 0.87 ± 0.24 （p 〈0.05）, which is larger than the group B（0.75 ±0.32）. The mitochondria in group B is different sizes, edema, were round, which have degeneration, severe visible ridge to reduce the fracture or disappear; The mitochondria in group C do not apparent damage. The ratio of PCNA-positive cells after 12h is 0.39 ± 0.16 （P〈0.05） in liver cells. Conclusion： polyene phosphatidyl choline can protect liver cells, by protecting the mitochondria and promote liver cell proliferation, reduce liver injury. In rat liver after liver transplantation to restore a facilitator.
出处
《中国现代普通外科进展》
CAS
2013年第1期15-18,共4页
Chinese Journal of Current Advances in General Surgery
关键词
肝移植
多烯磷脂酰胆碱
大鼠
Liver transplantationo Polyene phosphatidylcholine· Rats
