摘要
目的探讨淫羊藿苷抑制骨吸收的机理。方法贴壁分离法培养大鼠骨髓基质干细胞,流式检测细胞纯度。加入10-5M淫羊藿苷12 h、24 h、36 h、48 h后用Real-time-RT-PCR检测OPG和RANKL基因表达,Western blotting检测蛋白表达。结果第2代培养细胞的纯度达到95%以上。OPG基因和蛋白表达水平在24 h时明显升高,与对照相比有显著差异。OPG和RANKL的比值显著高于对照组。结论淫羊藿苷主要通过提高OPG及OPG和RANKL的比值来发挥抑制骨吸收的作用。
Objective To investigate the mechanism of icariin on inhibition of bone resorption. Methods Rat bone marrow stromal cells were isolated by adherence, and were identified by flow cytometry, mRNA level of OPG/RANKL was analyzed using quantitative real-time RT-PCR. Protein level of OPG/RANKL was analyzed with Western blotting. Results Rat bone marrow stromal cells of passage 2 were more than 95% identical. The OPG mRNA and protein expression increased significantly at 24 h. The difference was significant compared to the control. The ratio of OPG/RANKL was markedly increased compared to the control. Conclusion Icariin inhibits bone resorption through increasing OPG and OPG/RANKL ratio,
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2013年第1期1-5,共5页
Chinese Journal of Osteoporosis
基金
甘肃省科技重大专项资助项目(09ZNKDA025)
