摘要
目的研究清热消积方对体外肿瘤细胞诱导的人脐静脉内皮细胞增殖凋亡的影响。方法将不同浓度清热消积方与人肺腺癌细胞SPC-A-1和人脐静脉内皮细胞HUVEC共同作用,用MTT法检测清热消积方对SPC-A-1及HUVEC细胞增殖的影响;流式细胞术检测对HUVCE细胞周期及凋亡的影响。结果不同浓度的清热消积方能够抑制SPC-A-1及HU-VEC细胞的增殖(P<0.01),呈现一定的剂量依赖性;并对两者存在差异细胞毒作用,对HUVEC细胞的增殖抑制显著高于SPC-A-1细胞(P<0.01);可将SPC-A-1细胞诱导的HUVEC细胞阻滞于细胞周期G2～M期;并引起HUVEC细胞凋亡。结论清热消积方可抑制SPC-A-1及HUVEC细胞的增殖、阻滞SPC-A-1诱导的HUVEC细胞周期,并引起该细胞凋亡,清热消积方可能具有抗肿瘤血管形成效应。
Objective To study the proliferation and apoptosis effect on human umbilical vein endothelial cell（HUVEC） induced by human lung adenocarcinoma cells（ SPC - A - 1 ） treated with Qingrexiaoji Recipe. Methods SPC - A - 1 and HUVEC cell were treated together with different concentrations of Qingrexiaoji Recipe, then we used MTT method to test the proliferation effect on both cells, and used flow cytometry to test the effect on cell cycle and apoptosis on HUVEC. Results Different concentrations of Qingrexiaoji Recipe had inhibit effection on proliferation of SPC - A - 1 and HUVEC cells, and showed a dose - effect dependent curve, which was different and notable on HUVEC cell（P 〈 0.01 ）. The Recipe can also block the cycle of HUVEC cell which induced by SPC - A - 1 at G2 - M period, then induce cell apoptosis. Conclusion Qingrexiaoji Recipe can inhibit the proliferation of SPC - A - I and HUVEC cells, block the cycle of HUVEC cell which induced by SPC - A - 1, then induce the cell apoptosis, which indicates that the recipe may probably have effect of anti tumor angiogenesis.
出处
《医学研究杂志》
2013年第1期148-151,共4页
Journal of Medical Research
基金
浙江省教育厅重点研究项目(Z2009-07128)
