摘要
目的研究血瘀型非酒精性脂肪性肝病氧化应激水平动态变化的特征。方法 SD大鼠随机分成正常组10只、NAFLD组及血瘀型NAFLD组各30只;正常组大鼠以标准饲料喂养,其他两组以高脂饲料喂养,血瘀型NAFLD组在高脂饲料喂养同时联合NE加BSA复合因素造模。正常组全部在12周末处死,后两组大鼠分别在实验开始后4、8及12周末3个时相点分批随机处死各10只。采用硫代巴比妥酸法测定血清及肝组织匀浆MDA含量、黄嘌呤氧化酶法测定SOD水平、二硫代硝基苯甲酸法测定GSH-PX水平。结果 NAFLD和血瘀型NAFLD 4、8、12周时相点大鼠血及肝组织SOD、GSH均较正常组明显降低(P<0.01),MDA较正常组明显增高(P<0.01);血瘀型NAFLD 8、12周时相点大鼠血及肝组织MDA较同期NAFLD组明显增高(P<0.05);血瘀型NAFLD12周时相点大鼠血及肝组织SOD较同期NAFLD组明显降低(P<0.01);血瘀型NAFLD8周时相点血GSH-PX及8、12周时相点大鼠肝GSH-PX均较同期NAFLD组明显降低(P<0.05)。结论 NAFLD和血瘀型NAFLD大鼠氧化应激水平均随着造模时间的延长而不断增强;而同样造模时间血瘀型病证结合模型的氧化应激反应比单纯疾病模型强。
Objective To study the dynamic change of related indictors to oxidative stress in NAFLD rats with blood stasis. Methods SD rats were randomly divided into the normal group （ 10 rats） , the NAFLD group （30 rats） and the NAFLD with blood stasis group （ 30 rats）. Except for the rats in the normal group being fed with the normal diet, the rats in the other groups were fed with high - fat diet. Simultaneously, the rats in the NAFLD with blood stasis group were dealed with norepinephrine（NE） and bull serum albumin（BSA）. The normal rats were executed at the end of the 12th week, and the 10 rats in the NAFLD with blood stasis group were respectively executed at the end of the 4th ,8th and 12th week. The levels of serum and liver MDA ,SOD, GSH - PX were respectively examined with thiobarbituric acid method, xanthine oxidase method and two thia nitrobenzoic acid method. Results Compared with the normal rats, the levels of serum and liver SOD, GSH -PX in the NAFLD rats and NAFLD with blood stasis groups at the time phase points of 4th week, 8th week and 12th week were obviously decreased （ P 〈 0.01 ） , but the levels of serum and liver MDA were increased（ P 〈 0.01 ）. Compared with the corresponding NAFLD rats, the levels of serum and liver MDA increased（P 〈 0.05）at the time phase points of 8th week and 12th week; the levels of serum and liver SOD in the NAFLD with blood stasis group were obviously decreased at the time phase point of 12th week（ P 〈0.01 ）. Compared with the corresponding NAFLD rats, the levels of serum GSH - PX in the NAFLD with blood stasis group at the time phase point of 8th week and the levels of liver GSH -PX at the time phase points of 8th week and 12th week were obviously decreased（ P〈 0.05）. Conclusion The levels of related indictors to oxidative stress in NAFLD rats and NAFLD with blood stasis were gradually increased with the molding time. The level of related indictors oxidative stress in rats of blood stasis in NAFLD group was stronger than that in NAFLD group in the corresponding period.
出处
《医学研究杂志》
2013年第1期83-86,共4页
Journal of Medical Research
基金
浙江省实验动物科技计划项目(2009F80014)
关键词
非酒精性脂肪性肝病
血瘀证
氧化应激
大鼠
病证结合
NAFLD
Blood stasis syndrome
Oxidative stress
Rat
Model combined disease with syndrome
