摘要
以大型观叶植物鹿角蕨(Platycerium wallichii Hook.)为材料,采用无菌培养和常规繁殖方法,研究其孢子培养及幼苗的复壮。结果表明:孢子萌发适宜温度为 20 ～ 30 ℃;在黑暗条件下孢子不萌发,孢子萌发和配子体发育最适光照强度为 60 ～ 80 μmol · m-2· s-1;配子体在 pH 4.5 ～ 7.5 的范围都可以正常发育;蔗糖浓度小于等于 2%的培养条件更利于孢子的萌发及原叶体的形成。当幼孢苗发育到 2 ～ 3 cm时即可移栽,移栽适宜的基质为腐殖土︰河沙︰有机肥 = 4︰2︰1;幼孢苗复壮 4 个月左右,株高约 10 cm时即具有一定的观赏价值。
The large ornamental plant，Platycerium wallichii Hook.，was investigated with both axenic cultivation and soil medium to study its spore culture and rejuvenation. The results indicated that the optimal temperature of spore germination are about 20–30 ℃. Spores did not germinate in dark conditions，and the optimum light intensity for growth are 60–80 μmol · m-2 · s-1. The successful development of gametophyte was achieved with the pH 4.5 to 7.5. It is more suitable for spore germination and prothallus to formation with 2% or lower sucrose. When the young sporophytes reach 2–3 cm length，it can be transplanted. The young sporophyte can be cultivateded in the mixed medium composed of leaf mould substrate，sand and organic fertilizer. Young sporophyte shows somewhat ornamental values when it has 10 cm height.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第1期155-162,共8页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(31170294)
关键词
鹿角蕨
孢子
有性繁殖
Platycerium wallichii Hook, spore, sexual propagation
