摘要
目的观察前列腺术后犬前列腺部尿道再上皮化的修复过程，并探讨基底层细胞角蛋白（CK）34（CK3413E12）细胞在再上皮化过程中的作用。方法选择15只老年家犬行2-μm激光前列腺汽化切除术，分别于术后3、7、14d序贯取材，采用免疫组织化学染色法检测前列腺部尿道上皮细胞CK34、前列腺特异抗原（PSA）表达水平。结果术后3d手术创面未见CK34阳性表达细胞；术后7d可见连接成片的CK34阳性细胞覆盖手术创面，且这些细胞与残余前列腺组织连续；术后14d新生尿道上皮的基底细胞层阳性表达CK34。正常或增生的前列腺组织可见前列腺腺泡、腺管上皮胞质PSA阳性，前列腺部尿道上皮PSA表达阴性。术后14d再生的前列腺部尿路上皮的PSA均为阴性表达。结论前列腺组织是前列腺增生症术后前列腺尿道再上皮化的重要组织，而CK34阳性的基底细胞可能是这一修复过程的主要细胞。
Objective To study the re-epithelialization of dog＇s prostate urethra after two-micron laser resection of the prostate （TmLRP） and the role of CK34 （CK3413E12）-positive cells in this process. Methods TmLRP were performed in 15 elder male dogs and wound specimens harvested at Days 3, 7 and 14 respectively. Immunohistochemical staining was performed to determine the expression levels of CK34 and prostate specific antigen （PSA） in prostatic urethra urothelial cells. Results There was no CK34-positive cells in surgical wounds after 3 days while a cluster of CK34-positive cells were found to cover surgical wounds after 7 days, and these cells attached to residual prostate tissues. At Day 14 post-operation, positive expression of CK34 was found in basal cells of newborn urothelium. Conclusion Prostate tissues are the important organizations for re-epithelialization of prostatic urethra after benign prostate hyperplasia surgery, and CK34-positive basal Cells play an important role in this process.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2013年第4期261-264,共4页
National Medical Journal of China
基金
贵州省科技厅基金[黔科合人才团队（2012）4013、黔舅科合SY（2011）3024、黔科合SZ（2012）3077]
