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根尖终末工作宽度对根管内细菌清除效果的体外研究 被引量:2

In Vitro Evaluation of the Effectiveness of Intracanal Bacterial Removal Related to Apical Terminal Working Widths
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摘要 目的:比较不同的根尖预备终末工作宽度对根管内细菌清除效果的影响。方法:将已感染粪肠球菌的48颗离体上颌磨牙的远中颊侧根管标本随机分为6组,实验组分别预备根管至25#(A组)、30#(B组)、35#(C组)、40#(D组);对照组E、F组不进行机械预备而只进行冲洗。采用ProTaper机用镍钛锉和不锈钢K锉冠向下法预备根管。A、B、C、D、E组用1%次氯酸钠和17%EDTA冲洗根管,F组仅用生理盐水冲洗。根管预备前和预备后分别取样作细菌学培养计数,并做统计学分析。结果:预备冲洗后6组根管内的粪肠球菌量均显著下降(P<0.05),其中D组下降最明显。A、B组间差异无统计学意义,其余各组间比较均有显著性差异(P<0.05)。在实验组A、B、C、D组中,根管预备后阴性培养的百分率分别为0%、12.5%、37.5%和75%;对照组E、F组中均未获得阴性培养。结论:增大根尖预备终末工作宽度能提高根管内细菌清除的效果。但上颌磨牙远中颊侧根管预备至40#配合抗菌性冲洗液,尚不能完全清除根管内粪肠球菌。 Objective: To compare and evaluate the effect of intracanal bacterial removal related to different apical terminal working widths of apical preparation. Methods: Forty-eight distobuccal root canals of maxillary molars teeth inoculated with Enterococcus faecalis were randomly and evenly divided into six groups, which underwent different apical preparation sizes: group A was 25# , group B was 30# , group C was 35# , group D was40# , and group E and F were the control groups with root cananl irrigation and no mechanical instrumentation. Root canals were prepared with ProTaper rotary instruments and stainless steel K files using a crown-down technique. Group A,B,C,D,E were irrigated with 1% sodium hypochlorite and 17% EDTA;Group F was irrigated with 0.9% saline water. Microbiological samples were taken from the root canals before preparation and after preparation. The colony forming units (CFUs) were counted and analyzed. Results: The numbers of Enterococcus faecalis in root canals in each of the six groups were effectively reduced (P〈0. 05), and group D showed maximum reduction in CFUs. There was no statistically significant diference between group A and group B, while differences between other groups were significant (P〈0.05). In groups A,B,C and D, percentages of postpreparation negative cultures were 0%, 12.5% ,37.5% and 75%, respectively. In the control groups E and F, none of the specimens showed negative culture. Conclusion.. The effect of reducing intracanal bacterial was improved with progressively larger apical preparation size. But a complete elimination of the intracanal bacteria was not achieved when distobuccal root canals of maxillary molars teeth were prepared to apical size 40# with antibacterial irrigation.
作者 罗玉 杨健
出处 《口腔医学研究》 CAS CSCD 2013年第1期55-57,60,共4页 Journal of Oral Science Research
基金 江西省教育厅科学技术研究项目(编号:GJJ11342)
关键词 工作宽度 根尖预备 粪肠球菌 Working width Apical preparation Enterococcus faecalis
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参考文献11

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二级参考文献7

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共引文献7

同被引文献16

  • 1马净植,刘薇,陈卫民.不同锥度机用镍钛器械预备对根管壁清洁率的影响[J].临床口腔医学杂志,2007,23(10):611-612. 被引量:1
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