摘要
目的:研究血链球菌细菌素对白色念珠菌(C.a)及热带念珠菌(C.t)的抑制作用及形态学影响。方法:通过低温高速离心,超声破碎等方法提取血链素,使血链素分别作用于单独及混合培养的C.a及C.t,于37℃下80r/min振荡培养。培养12h时,以平板菌落计数法测定不同浓度血链素的抑菌活性;每2h测1次各组菌悬液的A值,并绘制生长曲线,观察血链素对C.a及C.t生长曲线的影响。在血链素对C.a及C.t的作用过程中,利用光学显微镜及扫描电镜对菌体形态进行观察。结果:单独及混合培养的C.a及C.t在1g/L血链素作用后菌落数低于对照组,差异有统计学意义(P<0.05)。血链素作用后,C.a及C.t的生长曲线在6～14h发生显著改变,对数生长期被推迟8h。血链素作用后,C.a及C.t的孢子及菌丝表面出现"盘状凹陷"形态。结论:血链素对单独及混合培养的C.a及C.t具有显著抑制作用。血链素使C.a及C.t的对数生长期推迟。血链素可导致念珠菌C.a及C.t的菌体形态发生改变。
Objective： To study the inhibition and morphological impact of Streptococcus sanguis bacteriocin on Candida albieans（C, a） and Candida tropicalis（C, t）. Methods： Streptococcus sanguis bacteriocin was extracted by low-temperature high-speed centrifugation, ultrasonic disruption, and then was respectively applied to separate and mixed cultured C. a and C. t under 37℃ 80rpm oscillating culture. At 12h, we used plate colony counting method to determine the antibacterial activity of Streptococcus sanguis bacteriocin with different concentrations. We measured each group bacterial suspension OD value every 2 hours, drew the growth curve, studied the effect of Streptococcus sanguis bacteriocin on growth curve of C. a and C. t. We observed morphology by optical microscopy and scanning electron microscopy in the process of cultivation. Results： After the inhibition of lg/L Streptococcus sanguis bacteriocin, the number of the colonies of separate and mixed cultured C. a and C. t were less than the control group. The difference was statistically significant. The growth curve of C. a and C. t changed significantly in 6 -14h, and the logarithmic growth phase was delayed 8 hours. Discoid depression was appeared on surface of spore and hyphae after inhibition. Conclusion.. Streptococcus sanguis bacteriocin could significantly inhibit separate and mixed cultured C. a and C. t, delay the logarithmic growth phase and change the morphology of C. a and C. t.
出处
《口腔医学研究》
CAS
CSCD
2013年第1期24-28,共5页
Journal of Oral Science Research
基金
黑龙江省自然科学基金(编号:D2007-03)
关键词
血链球菌细菌素
白色念珠菌
热带念珠菌
菌体形态
生长曲线
Streptococcus sanguis bacteriocin
Candida albicans
Candida tropicalis
Morphology
Growthcurve
