摘要
目的探讨钛表面固定具有诱导仿生矿化作用的活性寡肽,及其细胞相容性。方法利用生物化学方法制备类釉原蛋白寡肽/聚多巴胺修饰的纯钛片,使用扫描电镜、傅立叶变换红外光谱仪对修饰前后的钛片进行分析。全骨髓培养法培养SD大鼠骨髓基质干细胞(BMSCs),将第3代BM-SCs与不同修饰的钛片共培养,采用荧光染色、细胞计数试剂盒(CCK)-8检测法、碱性磷酸酶(ALP)活性检测评价此种修饰对BMSCs生长、增殖及分化的影响。结果类釉原蛋白寡肽/聚多巴胺共聚物已成功接枝到碱热处理后的钛片上,聚多巴胺预修饰及其与类釉原蛋白寡肽共同修饰的钛片对BMSCs生长、增殖及分化无明显影响,修饰后的钛片与BMSCs具有较强的亲和力。结论类釉原蛋白寡肽/聚多巴胺共价接枝有望成为一种合适的钛表面仿生修饰方法,这为研究钛及其合金在复杂的生理环境中实现仿生矿化奠定实验基础。
Objective To immobilize active oligopeptide with the effect of inducing biomimetic mineralization on the titanium surface via biochemical methods and to evaluate its cell compatibility. Methods The amelogenin-in- spired oligopeptide/polydopamine-modified titanium plates were made using biochemical methods, and titanium plates before and after the modification were characterized by means of SEM and FTIR. SD rats bone marrow stro- mal stem cells （BMSCs） were cultured using the method of whole bone marrow. The third passage of BMSCs were cocuhured with different modified titanium plates, fluorescence staining, CCK-8 method and alkaline phosphatase activity detection were taken to evaluate the effect of such modification on the growth, proliferation and differentiation of BMSCs. Results The amelogenin-inspired oligopeptide/polydopamine copolymer had been successfully grafted onto the alkali-heat treated titanium plates. The polydopamine pre- modified titanium pieces and its common modified titanium pieces with amelogenin-inspired oligopeptide had no significant effects on the growth, proliferation and differentiation of BMSCs, and modified titanium plates had a strong affinity for BMSCs. Conclusion The amelogenin-inspired oligopeptide/polydopamine covalently bond is expected to become an appropriate bionic modifi- cation for titanium surface,which will lay an experimental foundation for studying the biomimetic mineralization of titanium and its alloys in a complex physiological environment.
出处
《安徽医科大学学报》
CAS
北大核心
2013年第2期124-127,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省高等学校自然科学基金重点项目(编号:KJ2009A169)
关键词
类釉原蛋白寡肽
多巴胺
钛
表面修饰
骨髓基质干细胞
amelogenin-inspired oligopeptide
dopamine
titanium
surface modification
bone marrow stromal stem cells
