摘要
对3个通过基因枪转化GUS基因获得的小麦条锈菌毒性突变体,进行GUS基因的表达活性检测、插入片段DNA的PCR电泳检测和Southern杂交验证;同时用17个国内鉴别寄主和37个黄淮麦区小麦主栽品种对这3个突变菌株进行了毒性谱的测定。结果表明突变菌株的外源GUS基因可稳定遗传;插入的外源基因片段引起了菌株毒性谱的改变。外源基因的插入位点与条锈菌的毒性基因相关。研究还表明插入突变体具有相对的遗传稳定性。研究结果可为克隆小麦条锈菌毒性基因和探明毒性基因的作用机理奠定理论基础。
In the previous study, 3 virulence mutants of wheat stripe rust pathogen, Puccinia striiformis, with GUS insertion were obtained. In this study, expression of GUS gene was analyzed by histochemical staining with X-Gluc, and confirmed by specific PCR and Southern blot. The results indicated that the alien gene GUS was integrated and could inherit stably in the stripe rust genome. The pathogenicity of the 3 mutants on 17 wheat stripe rust differentials and 37 extension cultivars was different from that of the wild isolate. The inserted alien fragment resulted in the pathogenic variation of the mutants. The insertion sites of foreign gene were relevant to the virulent gene of Puccinia striiformis. This study provides a basis for the cloning of the avirulent genes.
出处
《植物病理学报》
CAS
CSCD
北大核心
2013年第1期42-49,共8页
Acta Phytopathologica Sinica
基金
国家自然科学基金(31101396)
国家"863"计划"农林有害生物分子生态调控技术(2012AA101503)
公益性(农业)行业科技专项(200903035)
高等学校学科创新引智计划项目(B07049)
关键词
小麦条锈菌
突变
SOUTHERN杂交
GUS
Puccinia striiformis f. sp. stritici West.
mutation
Southern blot
β-glucuronidase
