摘要
利用亲和毛细管电泳法研究了不同形态铬离子与牛血清白蛋白(BSA)结合的反应机制并进行了比较分析.模拟生理条件下,构建配体Cr(Ⅲ)、Cr(Ⅵ)与受体(BSA)相互作用模型,依据BSA有效淌度的变化,通过非线性模拟方程计算Cr(Ⅲ)-BSA和Cr(Ⅵ)-BSA结合反应的表观结合常数KCr(Ⅲ)-BSA、KCr(Ⅵ)-BSA,定量表征Cr(Ⅲ)、Cr(Ⅵ)与BSA结合反应的差异性.结果表明,Cr(Ⅲ)、Cr(Ⅵ)与BSA的结合反应与金属离子形态之间存在明显的价态相关性,而同一形态金属离子随着Cr(Ⅲ)、Cr(Ⅵ)浓度的变化与BSA均存在量效关系,同时通过解析电泳谱图获得了Cr(Ⅲ)、Cr(Ⅵ)与BSA结合反应均为快平衡体系的结论.
The binding reaction mechanism between different forms of chromium ion and bovine serum albumin （BSA） was investigated using affinity capillary electrophoresis （ACE） method, and a comparative analysis was carried out. An interaction model of the ligand （Cr（ Ⅲ ） and Cr（ Ⅳ ）） binding with the receptor （BSA） was established under simulated physiological conditions. Based on the changes of effective mobility of BSA, the apparent binding constants of Cr（ Ⅲ ）-BSA and chromium（ Ⅳ ）-BSA were calculated by a nonlinear fitting equation and the differences of binding reaction between chromium （ Ⅳ ） and chromium （ Ⅳ ） with BSA was quantificational characterized. The results showed that the obvious valence correlation existed between the binding reactions of Cr Ⅲ）, Cr （VI） and bovine serum albumin （BSA）, and with the changes of concentrations of Cr（ Ⅲ ） and Cr（Ⅳ ）, the obvious dose-effect relationship existed between metal Cr irons with BSA. A conclusion about a quick balance system of the binding reaction between Cr（Ⅲ） and Cr（ Ⅳ ） with BSA was also acquired by analyzing electrepherogram. This work has a referential meaning for the further analysis of the binding reaction mechanism of different metal iron forms and protein molecule.
出处
《环境化学》
CAS
CSCD
北大核心
2013年第1期65-70,共6页
Environmental Chemistry
基金
国家自然科学基金项目(20877072)资助
关键词
亲和毛细管电泳
Cr(Ⅲ)
Cr(Ⅵ)
牛血清白蛋白
结合反应
affinity capillary electrophoresis, chromium （Ⅲ ） , chromium （ Ⅳ）, bovine serum albumin,binding reaction.
