摘要
目的研究雾化吸入与肌内注射干扰素α1b(IFNα1b)在兔体内分布及代谢的差异,考察雾化吸入干扰素治疗肺部疾病的优越性。方法将干扰素α1b进行125I标记,选用新西兰大耳白兔,以肌内注射为对照,采用雾化吸入的方式给药,不同时间点取血,γ计数仪测定血药浓度,通过小动物活体成像系统和γ计数仪检测肺组织不同部位的药物分布情况和主要代谢脏器肝和肾中的药物含量。结果与肌内注射比较,雾化吸入给药后肺组织中的干扰素含量更高,肺组织内滞留时间长,血药浓度达峰时间为8 h,明显延迟,而肾组织中的药物含量始终维持较低浓度。结论与常规肌内注射给药相比,雾化吸入干扰素α1b肺组织药物含量高,药物体内循环时间长,是干扰素治疗肺部疾病更为有效的给药途径。
Objective The variety on distribution and metabolism between aerosol inhalation and intra-muscular injection of interferon-crib（IFNαdb） in rabbits were studied. The priority of IFNcdb administration via aerosol inhalation was investigated. Methods The IFNodb was labeled with iodine-125 and new Zealand white rabbits were used. The 125HFNαdb was administered via aerosol inhalation, with intramuscular injection as control. Blood was collected at different time and drug concentration in blood was detected by Gamma counter, the drug distribution in different part of lung and metabolic tissues like liver and kidney was imaged by in vivo imaging system and detected by Gamma counter. Results The drug concentration was higher and drug residence time was longer in lung after administered via aerosol inhalation than those with intramuscular injection. The blood drug concentration reached maximum after being administered for 8 hours, and which significantly prolonged. But the drug concentration in kidney was kept at low level within detected time. Conclusion The interferon-crib administered via aerosol inhalation keeps higher drug concentration in lung and longer circulation time in body, comparing with the conventional intramuscular injection, which suggests that the aerosol inhalation may be a more effectively administered way for treating lung disease.
出处
《医药导报》
CAS
北大核心
2013年第1期1-5,共5页
Herald of Medicine
基金
国家自然科学基金资助项目(81171371,30700178)
放射医学研究所学科发展基金(所发1209)
关键词
干扰素Α1B
雾化吸入
125I标记
Interferon-α1b
Aerosol inhalation
Iodine-125 radiolabeling
