摘要
为探索蛇床子素的提取捡测方法,通过正交试验方法对蛇床子素的提取工艺进行优化研究。结果表明:蛇床子的最佳提取工艺为用5倍量95%乙醇55℃水浴震荡提取3次,每次3h,提取量为8.463mg.g-1。该研究建立了蛇床子素的高效液相色谱法检测方法,色谱条件:流动相,色谱甲醇∶水(60∶40);柱温35℃;紫外检测器检测波长322nm;进样量10μL;流速1.0mL.min-1。该方法能够准确地对蛇床子素进行定性和定量检测。
In order to explore the detection method to extract osthol,the optimal extraction process of the Frutus cnidii was determined through orthogonal experiments. The results showed that shocking and extracting it 3 times with 5 times of the amount of 95 % ethanol in 55＇C water bath,3 h each time,the extraction amount was 8. 463 mg . g -1. The detecting method of osthol was also discussed with HPLC,chromatographic condition： mobile phase,chromatographic pure methanol and water（60： 40） ;column temperature was 35℃ ;and extra-purple detector was used, detection wave length was 322 nm; the injection volume was 10 μL, flow rate was 1.0 mL.min-1. The experiment indicated that the method could be used to detect the osthol exactly.
出处
《黑龙江农业科学》
2013年第2期104-106,共3页
Heilongjiang Agricultural Sciences
