摘要
采用Acquity UPLC HSS T3色谱柱,以乙腈-10 mmol/L乙酸铵为流动相,0.2 mL/min梯度洗脱方式辅以电喷雾离子源正离子模式多反应监测(MRM)进行定性分析,建立了一种快速高效液相色谱-串联质谱(RRLC-MS/MS)同时测定19种核苷的分析方法。19种核苷的定量限及检出限分别在0.05～20μg/L和0.02～10μg/L之间;其线性相关系数均大于0.99,且线性范围满足实验要求,回收率为79.01%～119.76%;标准偏差均小于14%。本方法快速、灵敏、稳定。将本方法应用于大肠杆菌中核苷的检测,在不同生长时期的大肠杆菌中检测到7种核苷。进一步的分析显示,随生长时间的延长,大肠杆菌胞内鸟嘌呤核苷含量显著下降。
A sensitive and selective method was developed for quantitation of nucleosides. The method included a UPLC HSS T3 column separation operated with a mobile phase of 10 mmol/L ammonium acetate and acetonitrile at a flow-rate of 0.2 mL/min coupled to a mass spectrometry scanned in multiple reaction monitoring （MRM） mode. In the result, the limits of quantification and detection of 19 nucleosides were in the range of 0.05-20 ~tg/L and 0.02-10 ~g/L, respectively. The linearity of the detected nucleosides was excellent with R2〉 0.99 and the limits of detection were all satisfied. The recoveries of standard additions for the compounds were between 79.0!% and 119,76%, and the relative standard deviation was below 14%. The method was capable of quantitation for 7 nucleosides in Escherichia coli, of which guanosine was found to decrease along with culture phases.
出处
《分析化学》
SCIE
CAS
CSCD
北大核心
2013年第1期36-41,共6页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金项目(No.20776029)
辽宁省教育厅重点实验室项目(No.LS2010012)资助
关键词
液相色谱-串联质谱
核苷
大肠杆菌
Rapid resolution liquid chromatography-tandem mass spectrometry
Nucleoside
Escherichia coli
