摘要
目的:建立HPLC同时测定口炎清颗粒中7个有机酸(新绿原酸、绿原酸、隐绿原酸、咖啡酸、3,4-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸及4,5-二咖啡酰奎宁酸)含量的方法。方法:采用AkzoNobel Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,柱温30℃,以乙腈-0.1%磷酸水溶液进行梯度洗脱,流速1.0 mL·min-1,检测波长326 nm。结果:7个成分的线性关系良好,精密度、稳定性、重复性的RSD均低于3%,加样回收率为97.75%～99.33%。结论:该方法可用于口炎清颗粒多指标质量控制。
Objective：To establish an HPLC method for the simultaneous determination of 7 organic acids（neochlo- rogenic acid, chlorogenie acid, eryptochlorogenic acid, caffeic acid, 3,4 - dicaffeoylquinic acid, 3,5 - dicaffeoylquin- ic acid and 4,5 - dieaffeoylquinic acid） in Kouyanqing granules. Methods：The chromatographic separation was per- formed on a AkzoNobel Kromasil C18 （250 mm × 4.6 mm,5μm）column, the column temperature was maintained at 30 ℃. A gradient elution of acetonitrile -0. 1% phosphoric acid aqueous solution was adopted at the flow rate of 1 mL . min-1. The UV detection wavelength was 326 nm. Results：All the 7 compounds showed good linearity in the range of the test concentration. The RSDs of the precision, reproducibility and stability tests were less than 3%, the average recoveries of them were in the range of 97.75% -99.33 %. Conclusion：The method can be applied for the multi - indictor quality control of Kouyanqing granules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第1期108-111,共4页
Chinese Journal of Pharmaceutical Analysis
基金
泸州市2010年重点科技项目(泸市财企[2010]41号)
泸州医学院2010年青年基金(泸医院[2010]108号)
关键词
高效液相色谱
口炎清颗粒
新绿原酸
绿原酸
隐绿原酸
咖啡酸
二咖啡酰奎宁酸
质量控制
标准提高
中药制剂
HPLC
Kouyanqing granules
neochlorogenic acid
chlorogenic acid
cryptochlorogenic acid
caffeicacid
dicaffeoylquinic acid
quality control
standard improvement
Chinese medicine preparation
