摘要
目的:用HPLC同时测定苍耳子中新绿原酸、绿原酸、隐绿原酸、噻嗪双酮苷、1,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸6个化学成分的含量。方法:采用Eclipse XDB-C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-0.1%磷酸为流动相进行梯度洗脱,流速1 mL·min-1,检测波长254 nm,柱温35℃。结果:新绿原酸、绿原酸、隐绿原酸、噻嗪双酮苷、1,5-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸色谱峰分离良好。进样量分别在0.012～1.2、0.028～2.8、0.012～1.2、0.014～1.4、0.024～2.4、0.011～1.1μg范围内线性关系良好。平均回收率(n=6)分别为102.3%、96.4%、99.1%、103.1%、103.0%、103.4%,RSD分别为1.8%、1.1%、1.8%、1.5%、2.0%、1.9%。结论:建立的苍耳子HPLC含量测定方法可用于苍耳子的质量控制。
Objective：To establish a method for simultaneous determination of 6 constituents （neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, xanthiside, 1,5 -dieaffeoylquinic acid, and 4,5 -dicaffeoylquinic acid） in Fructus Xanthii. Methods：The assay was conducted on a Eclipse XDB -Cls column（250 mm ×4. 6 mm, 5μm） with methanol -0. 1% Ha PO4 as mobile phase. The flow rate was 1.0 mL.min- l, the detection wavelength was 254 nm and the column temperature was 35 ℃. Results：The correlation coefficient between concentration and chromatograph- ic peak area of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, xanthiside, 1,5 - dicaffeoylquinie acid, and 4,5 - dicaffeoylquinic acid was over 0. 9999 in the range of 0. 012 - 1.2,0. 028 - 2. 8,0. 012 - 1.2,0. 014 - 1.4,0. 024 - 2. 4,0. 011 - 1.1 μg, respectively. The average recoveries （ n = 6） of 6 constituents were 102. 3%, 96. 4% ,99. 1%, 103. 1%, 103.0%, and 103.4%, respectively ; RSDs were 1.8%, 1.1%, 1.8%, 1.5%, 2. 0%, and 1.9%, respectively. Conclusion： The established method can be used for the quality control of Fructus Xanthii.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第1期78-82,共5页
Chinese Journal of Pharmaceutical Analysis
基金
国家重点基础研究发展计划(973计划)课题(2009CB522804)
关键词
中药质量控制
苍耳子
新绿原酸
绿原酸
隐绿原酸
噻嗪双酮苷
1
5-二咖啡酰奎宁酸
4
5-二咖啡酰奎宁酸
高效
液相色谱
traditional Chinese medicine quality control
Fructus Xanthii
neochlorogenic acid
chlorogenic acid
cryptochlorogenic acid
xanthiside
1,5 - dicaffeoylquinic acid
4,5 - dicaffeoylquinic acid
HPLC
