摘要
目的:探讨孕期不同时间给予低蛋白饮食致宫内发育迟缓(IUGR)大鼠脑白质细胞凋亡的影响。方法:怀孕wistar大鼠分为对照组(C组)及孕早期(E组)、孕中期(M组)、孕晚期低蛋白饮食组(L组),测量脑重及体重,观察脑组织损伤,脑细胞凋亡及Caspase-3表达情况。结果:出生时体重:E、M组与C组比较差异有统计学意义(P<0.05);脑重:E、M、L组与C组比较差异有统计学意义(P<0.05)。E组出生时脑损伤病变较轻,2周时加重。细胞凋亡数量:出生时E、M、L组均明显高于C组,随年龄增加凋亡细胞数量逐渐减少,生后4周仅E组明显高于C组。Caspase-3的表达:出生时E、M、L组均明显高于C组,随年龄增加Caspase-3的表达逐渐减少,生后4周仅E组明显高于C组。结论:低蛋白饮食应用越早,脑白质细胞凋亡率越高,持续时间越长。Caspase-3活性增强参与了IUGR的病理过程,与细胞凋亡密切相关。
Objective：To explore the effect of low-protein diet on cerebral white matter apoptosis in rats with intrauterine growth retardation（IUGR） during different stages of pregnancy.Methods：The pregnant Wistar rats were divided into control group（group C）,low-protein diet during early pregnancy group（group E）,low-protein diet during middle pregnancy group（group M）,and low-protein diet during late pregnancy group（group L）;brain weight and body weight were measured,brain damage,brain cell apoptosis,and Caspase-3 expression were observed.Results：There was statistically significant difference in birth weight among group E,group M,and group C（P0.05）;there was statistically significant difference in brain weight among group E,group M,group L,and group C（P0.05）.Brain damage of rats after birth in group E was mild,then it aggravated at 2 weeks.The numbers of cell apoptosis at birth in group E,group M,and group L were statistically significantly higher than that in group C;the number of cell apoptosis decreased gradually with age;at 4 weeks after birth,only the number of cell apoptosis in group E was significantly lower than that in group C.The expression levels of Caspase-3 in group E,group M,and group L at birth were statistically significantly higher than that in group C;the expression level of Caspase-3 decreased gradually with age;at 4 weeks after birth,only the number of cell apoptosis in group E was significantly lower than that in group C.Conclusion：The earlier low-protein diet applies,the higher apoptotic rate of brain white matter is,and the longer the duration time was.Enhancement of Caspase-3 activity participates in pathogenesis of IUGR,which is closely correlated with cell apoptosis.
出处
《中国妇幼保健》
CAS
北大核心
2013年第3期535-539,共5页
Maternal and Child Health Care of China
基金
辽宁省教育厅科研项目〔L2010278〕
关键词
宫内发育迟缓
大鼠
低蛋白
凋亡
Intrauterine growth retardation
Rat
Low protein
Apoptosis
