摘要
目的:筛选出具有胆盐水解酶活性的乳酸菌,并通过动物实验验证其降血脂的效果。方法:通过琼脂平板测试法和高效液相色谱法(HPLC)对乳杆菌胆盐水解酶活力进行定性和定量测试,筛选出具有胆盐水解酶活性的乳杆菌灌胃高脂血症大鼠;将雄性Wistar大鼠24只按体质量约相等分为正常对照组、高脂模型组及实验组,后两组给予高脂饲料喂养,并每日一次给这3组分别灌胃0.9%生理盐水、脱脂乳和活菌制剂,28d后测定相关指标。结果:L.fermentum MGH13-1具有较高的胆盐水解酶活性,将其灌胃大鼠后,实验组血清总胆固醇、低密度脂蛋白胆固醇、甘油三酯等含量明显低于高脂模型组(P<0.05),肝脏中的胆固醇含量明显低于高脂模型组(P<0.05),粪便中的胆固醇、胆酸及短链脂肪酸含量明显高于高脂模型组(P<0.05)。结论:具有胆盐水解酶活性的L.fermentumMGH13-1对高脂血症大鼠有明显的降血脂作用。
Objective： To screen Lactobacillus with bile salt hydrolase （BSH） activity and to explore its hypolipidemic effect in hyperlipidemic rats. Methods： Agar plate assay and high-performance liquid chromatography （HPLC） were used to detect and quantify bile salt hydrolase activity in Lactobacillus. Lactobacillus with excellent BSH activity was administrated by gavage to hyperlipidemic rats. Twenty-four male Wistar rats of similar body weight were divided into normal control, hyperlipidemia model and LactobaciIlus treatment groups. The latter two groups were fed high fat diet. Three groups were administrated by gavage with 0.9% normal saline, skim milk and skim milk inoculated with Lactobacillus once daily for 21 consecutive days and fasted overnight after the last administration. On the following day, blood and livers were taken for the determination of serum lipid indicators. Besides, rat stools were collected during the last three days of administration and tested for TC, total bile acid （TBA） and short-chain fatty acid （SCFA） levels. Results： Lactobacillusfermentum MGH13-1 showed excellent BSH activity. Serum ofTC, TG and LDL-C levels and liver TC level were significantly lower, while fecal TC, TBA and SCFA levels were significantly higher in MGH13-1 treatment group, when compared to high fat model group （P 〈 0.05）, Conclusion： Lactobacillus MGH 13-1 has a remarkable hypolipidemic effect in hyperlipidemic rats.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第1期257-262,共6页
Food Science
基金
国家自然科学基金项目(30840011
31160315)
中国科学院西部之光人才培养计划项目
内蒙古农业大学博士启动基金项目(BJ08-18)
内蒙古自然科学基金项目(200508010406
2011MS1205
20102010)
