摘要
目的观察纳米纤维载体／胶原凝胶复合体外肝细胞培养效果。方法超声振荡制备胶原／丝素纳米纤维载体，与胶原凝胶复合体外培养人肝癌HepG2细胞12d，测定细胞功能。结果纳米纤维载体，皎原凝胶复合组细胞聚集于纳米纤维载体周围生长，细胞功能持续增高于第9天达到峰值后下降；胶原凝胶培养组中细胞均匀分，细胞功能至第3天达到峰值后迅速下降。结论纳米纤维载体结合胶原凝胶复合体外肝细胞培养有利于维持肝细胞的白蛋白分泌功能及细胞活性，在生物人工肝中有一定的可行性。
Objective To investigate cell＇s viability and functions when human hepatocyte cell HepG2 and microcarriers were embedded in collagen gel and cuhured in vitro. Methods Ultrasound concussion crushing method was used to get nanofiber microcarrier. HepG2 and microcarriers were embedded in collagen gel and cultured in 12 d. The albumin （ALB）,urea and lactate dehydrogenase （LDH） levels in the nutrient solution were measured. Results The cells in nanofiber nanofiber microcarrier/collagen gel group were gathered around the microcarrier and formatted aggregates, the ALB and urea levels increased steadily and reached maximum in day 9, then decreased; In collagen gel without nanofiber microcarrier group cells uniformly distributed, the ALB and urea levels reached maximum in day 3, then decreased rapidly, and on day 6 majority of cells dead. Conclusion Gel entrapped nanofiber microcarriers and hepatoeytes is a high-density and longtime culture method which can possibly be used in the bioartificial liver system.
出处
《国际生物医学工程杂志》
CAS
2012年第6期321-324,共4页
International Journal of Biomedical Engineering
基金
东南大学生物电子学国家重点实验室开放研究基金项目
关键词
纳米纤维载体
肝细胞
胶原凝胶
细胞培养
Nanofiber microcarrier
Hepatocytes
Collagen gel
Cell culture
Bioartificial liver
