摘要
目的探讨瑞芬太尼对。肾脏缺血再灌注（I／R）损伤大鼠核苷酸结合寡聚化结构域1（NOD1）mRNA表达的影响。方法健康成年雄性SD大鼠60只，体重220～250g，采用随机数字表法，将其随机分为3组（n=20）：假手术组（S组）、缺血再灌注组（I／R组）和瑞芬太尼组（R组）。I／R组和R组采用夹闭双侧。肾动脉45min再灌注法制备肾脏I／R损伤模型。R组于缺血前15min时静脉输注瑞芬太尼1．0μg·kg^-1·min^-1至再灌注30min，S组和I／R组用等容量生理盐水替代。于缺血前15min及再灌注3、6、24h时取肾组织，观察。肾组织病理学结果，并计数中性粒细胞（PMN），采用流式细胞术舣染法检测细胞凋亡率，采用RT—PCR法测定NOD1mRNA表达水平。结果与S组比，I／R组再灌注各时点肾组织NOD1mRNA表达水平上调，细胞凋亡率和PMN计数升高，R组再灌注各时点肾组织细胞凋亡率和PMN计数升高，再灌注6、24h时NODlmRNA表达上调（P〈0．01）；与I／R组比较，R组再灌注各时点肾组织NOD1mRNA表达水平下调，细胞凋亡率和PMN计数降低（P〈0．05或0．01），且肾组织病理学损伤减轻。结论瑞芬太尼通过下调NOD1mRNA表达，抑制炎性反应和细胞凋亡，从而减轻大鼠肾脏I／R损伤。
Objective To investigate the effect of remifentanil on nucleotide-binding oligomerization do- main I （NOD1） mRNA expression in rats with renal ischemia-reperfusion （I/R） injury. Methods Sixty male Sprague-Dawley rats, weighing 220-250 g, were randomly divided into 3 groups （ n = 20 each） ： sham operation group （S group）, I/R group and remifentanil group （R group）. Renal ischemia was induced by occlusion of bilat- eral renal arteries for 45 min followed by 24 h reperfusion in groups I/R and R. Remifentanil 1.0 μg·kg^-1·min^-1 was infused until 30 min of reperfusion starting from 15 min before ischemia in group R, while the equal volume of normal saline was given instead in S and I/R groups. The animals were sacrificed at 15 min before ischemia and at 3, 6, 24 h of reperfusion and the kidneys were removed for microscopic examination and polymorphonuclear leuko- cyte （PMN） count and for measurement of NOD1 mRNA expression （by RT-PCR）. The apoptotic rate was deter- mined by flow cytometry double staining method. Results Compared with group S, NOD1 mRNA expression was up-regulated, and the apoptotic rate and PMN count were significantly increased at each time point during reperfu- sion in group I/R, and the apoptotic rate and PMN count were significantly increased at each time point during reperfusion, and NOD1 mRNA expression was up-regulated at 6 and 24 h of reperfusion in group R （ P 〈 0.01 ）. Compared with I/R group, NOD1 mRNA expression was down-regulated, and the apoptotic rate and PMN count were significantly decreased at each time point during reperfusion （ P 〈 0.05 or 0.01 ）, and the pathological changes were significantly attenuated in group R. Conclusion Remifentanil can reduce the renal I/R injury bydown-regulating the expression of NOD1 mRNA and inhibiting inflammatory response and apoptosis.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2012年第11期1393-1396,共4页
Chinese Journal of Anesthesiology
基金
基金项目：河北省科学技术研究与发展计划项目（10206133D）
