摘要
目的建立偏瘫复原胶囊中川芎和当归的主要成分阿魏酸含量测定方法。方法以Diamonsil C18柱(250 mm×4.6 mm,5μm)为色谱柱,70%甲醇-0.1%磷酸水溶液(30∶70)为流动相,检测波长为320 nm,流速为1.0 mL/min,柱温为30℃。结果阿魏酸质量浓度在0.1～100μg/mL范围内与峰面积呈良好线性关系,r=1.000;样品平均加样回收率为97.39%,RSD=0.83%(n=5)。结论该方法简便、准确,结果稳定,可用于偏瘫复原胶囊中川芎和当归的主要成分阿魏酸的测定。
Objective To establish a mothod for ferulic acid of Chuanxiong and Angelica in Hemiplegia Fecovery Capsules.Methods The method was adopted for the determination with Diamonsil C 18（250 mm × 4.7 mm,5 μm） as chromatographic column,70% methanol-0.1% phosphoric acid solution（30 ∶ 70） as mobile phase;the wavelength was 320 nm.The flow rate was 1.0 mL/min and the column temperature was at 30 ℃.Results The linear range of ferulic acid were 0.1 ～ 100 μg/mL（r = 1.000）,with average recoveries of 97.39%（n = 5）,RSD = 0.83%.Conclusion The method is simple,accurate and stable,which could be used for the quality control of Hemiplegia recovery capsules.
出处
《中国药业》
CAS
2013年第1期19-20,共2页
China Pharmaceuticals
关键词
瘫复原胶囊
阿魏酸
含量测定
Hemiplegia Recovery Capsule
ferulic acid
content determination
