摘要
目的:通过研究益气养血温阳方对泻药性便秘大鼠结肠Cajal间质细胞含量的影响,阐明益气养血温阳方治疗泻药性便秘的机制。方法:大鼠按照200 mg.kg-1.d-1大黄免煎剂灌胃,再按每日200 mg.kg-1.d-1递增,至半数以上动物出现腹泻,如此反复用药3月,造成"泻药性便秘"动物模型。将60只SD大鼠随机分为空白对照组、阳性对照(福松1.65 g.kg-1)组、模型组、益气养血温阳方低、中、高剂量(8.35,16.7,33.4 g.kg-1)组。阳性对照组、模型组、益气养血温阳方低、中、高剂量组给予大黄建立便秘大鼠模型,阳性对照和益气养血温阳方低、中、高剂量组按剂量给予药物治疗2周,取大鼠结肠组织,采用RT-PCR方法检测结肠中Cajal间质细胞表达。结果:益气养血温阳方高剂量组结肠组织Cajal细胞mRNA相对表达量为0.365 08±0.047,中剂量组为0.370 97±0.040,与模型组大鼠结肠组织中表达为0.298 55±0.046比较,有显著差异(P<0.05)。结论:益气养血温阳方通过改变Cajal间质细胞表达,促进结肠运动,起到治疗便秘的作用。
Objective： To study the effects and mechanism of Yiqi Yangxue Wenyang formula on interstitial cells of cajal in the rat colon of constipation caused by laxatives. Method： The rat constipation model was caused by laxatives on. First day rats were ig given rhubarb 200 mg·kg-1·d-1, second day given 400 mg· kg·kd-1 for 3 months, until half rats appeared diarrhea. The modelling rats were treated with Yiqi Yangxue Wenyang formula for two weeks, interstitial cells of cajal in colon was observed by RT-PCR. Result： The interstitial cells of cajal in rat colon in the Yiqi Yangxue Wenyang formula high dose group was 0. 365 08 ± 0. 047, the medium group was 0. 370 97± 0. 040, that was higher than that in the model group is 0. 298 55 ± 0. 046 （ P 〈 0. 05 ）. Conclusion： The Yiqi Yangxue Wenyang formula can treat constipation by adjust interstitial cells of cajal in colon.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第2期219-222,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
2009年四川省教育厅课题(09ZA021)
关键词
便秘
CAJAL间质细胞
益气养血
温阳通便
constipation
interstitial cells of cajal
tomifying qi and blood
Warming yang to promot defecation
