摘要
目的:建立秦艽花的液质联用指纹图谱检测方法,为最终实现秦艽花真伪品的鉴别提供参考。方法:以Agilent ZORBAX ECLIPSE XDB-C18(2.1 mm×50 mm,1.8μm)为分析用色谱柱,0.1%甲酸水溶液-乙腈为流动相进行梯度洗脱,在正离子模式下,分析11批秦艽花药材,并利用中药色谱指纹图谱相似度评价系统进行了相似度计算。结果:建立了秦艽花液质联用指纹图谱的共有模式,标定了指纹图谱中的23个共有峰,11批药材的相似度均>0.90,并用HPLC-ESI-Q-TOF-MS技术对7个共有峰进行了指认。结论:方法精密度、稳定性和重复性良好,为提高秦艽花的质量标准提供了依据。
Objective： To establish the fingerprint ehromatograms of Flos Gentianae Stramienae by the means of liquid chromatography-mass spectrography （ LC-MS）. Method： The analysis was performed on Agilent ZORBAX ECLIPSE XDB-C,s column （2. 1 mm × 50 mm, 1.8μm） with the mobile phase consisting of 0. 1% formic acid-water-acetonitrile in a gradient mode. The chromatograms were recorded by using ESI-MS in positive ion model. Totally, 11 batches of samples were analyzed and their similarities we＇re calculated using fingerprint similarity evaluation software. Result： The LC-MS fingerprint common mode of Flos Gentianae Stramienae was obtained. A total of 23 characteristic peaks were selected as the common fingerprint peaks. The similarities of 11 samples were greater than 0.90 as compared to the common mode and seven peaks were identified by using HPLC- ESI-Q-TOF-MS. Conclusion： The reported method is precise, reproducible and reliable. The establishment of LC- MS fingerprint chromatograms can be helpful for the quality control of Flos Gentianae Stramienae.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第2期67-70,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
秦艽花
指纹图谱
液质联用
龙胆苦苷
Flos Gentiana.e Stramienae
fingerprint
LC-MS
gentiopicroside
