摘要
本文报道用两种体外免疫及体外强化的方法,对7型腺病毒(7—AV)诱导BALB/c小鼠B淋巴细胞活化进行了研究。结果表明:活7—AV免疫后使培养的脾细胞存活率下降,适当浓度的灭活7—AV免疫可以使培养细胞增生并能在离体培养条件下存活2周,细胞存活率可达90％以上。巨噬细胞预先处理抗原的体外免疫方法的免疫效果较好,如采用体外强化方法可以诱生较明显的特异性抗体反应。
Specific antibody against Type 7 adenovirus (7-AV) was induced by mouse spleen cells immunized in vitro or in vivo and boosted in vitro. Inactivated 7 -AV ae immunogen was better than activated 7-AV. Immunizing spleen cells with macrophages activated by 7-AV could improve the immunization effect.High titer antibody could be produced by immunization in vivo and boosted in vitro
出处
《白求恩医科大学学报》
CSCD
1991年第3期211-213,共3页
Journal of Norman Bethune University of Medical Science
关键词
腺病毒
体外免疫
B细胞
In vitro immunization Adenovirus Hybridoma
