摘要
目的通过改进螺旋剪法建立制备气管螺旋条的旋割法。方法 40只豚鼠,用旋割法和螺旋剪法制备离体豚鼠气管螺旋条,在Kreb's液中平衡孵育2 h后,以组胺histamine(浴槽浓度2.0×10-3g/L)和乙酰胆碱acetylcholine(浴槽浓度2.0×10-4g/L)引发气管螺旋条收缩,用BL420生物信号采集系统与张力传感器测定标本张力变化值。数据采用SPSS11.5软件在α=0.05的信度下进行t检验。结果 2 g负荷下,旋割法标本histamine引发收缩幅度是螺旋剪法制备标本的1.31倍,乙酰胆碱引发收缩幅度旋割法是螺旋剪法制备标本的1.208倍,经t检验,P<0.05,差异均具有显著性;旋割法标本,histamine引发2 g负荷标本收缩幅度是1 g负荷的1.48倍,乙酰胆碱引发2 g负荷标本的收缩幅度是1 g负荷的1.38倍,经t检验,P<0.05,差异均具有显著性;旋割法标本经hista-mine或acetylcholine激发收缩,洗净药物重复激发6次收缩幅度的RSD值分别为19.8%和19.1%,螺旋剪法标本经histamine或acetylcholine 6次重复引发诱发收缩幅度的RSD值分别35.3%和33.7%。结论与螺旋剪法制备气管螺旋条标本比较,旋割法制备螺旋条标本对收缩诱导剂histamine与acetylcholine的敏感性高,标本负荷以2 g较好,旋割法标本重复利用收缩幅度变化值较螺旋剪法标本小。
Objective To improve the preparation of trachea spiral strips of guinea pigs by spiral cutting using surgical scissors and to establish a new method of spiral cutting using surgical blade. Methods Forty guinea pigs were en- rolled in this experiment. By method of spiral cutting with surgical scissor or surgical blade to prepare isolated guinea pig trachea spiral strips, and incubate them in Kreb＇s solution for 2 hours. Contraction of the trachea spiral strips were induced by histamine in concentration of 2.0 ×10 -3 g/L or aeetylcholine in concentration of 2.0 × 10 -4 g/L. The changes of ten- sion values were measured using a BIA20 biological signal acquisition system and tension sensors. SPSS 11.5 software was used to analyze the data （ t test, a = 0.05 ）. Results Under 2 g tension, the contraction caused by histamine of the surgi- cal blade-prepared trachea strips was 1.31-fold higher than that of surgical scissors-prepared tracheal strips, and the con- traction caused by acetylcholine of the surgical blade-prepared trachea strips was 1.20g-fold higher than that of surgical scissors-prepared tracheal strips, both with a significant difference （ t test, P 〈 0.05 ）. The contraction amplitude causedby histamine of surgical blade-prepared specimens under 2 g tension was 1.48-fold higher than that caused under 1 g ten- sion, and the contraction amplitude of that caused by acetylcholine under 2 g tension was 1.38-fold higher than that under 1 g tension, both with a significant difference （t test, P 〈 0.05）. When histamine and acetylcholine were washed out from the tracheal strips, their tension returned to baseline. The RSD values of contraction amplitude of surgical blade-prepared strips were 19.8% （by histamine） and 19.1% （by acetylcholine）, and the RSD values of contraction amplitude of surgi- cal scissors-prepared strips were 35.3% （by histamine） and 33.7%. Conclusions Comparing the surgical blade-pre- pared tracheal spiral strips with those of surgical scissors-prepared ones, the former is more sensitive to histamine and ace- tylcholine treatment, and it is better to be treated under 2 g tension. The contraction variation caused by recycling of surgi- cal blade-prepared specimens is smaller than that of surgical scissors-prepared specimens.
出处
《中国实验动物学报》
CAS
CSCD
2012年第6期65-68,共4页
Acta Laboratorium Animalis Scientia Sinica
基金
2010年度甘肃省教育厅项目(No 1006B-10)
甘肃中医学院中青项目(No BH2010-079)
2011甘肃省中药药理与毒理学重点实验室(培育基地)开放基金(NoZDSYS-KJ-2012-01)支持
关键词
气管条
收缩
组织胺
乙酰胆碱
旋割法
螺旋剪法
豚鼠
Tracheal spiral strip
Contraction
Histamine
Acetylcholine
Preparation procedure, spiral cutting with surgical scissor
Preparation procedure, spiral cutting with surgical blade
Guinea pigs
