摘要
目的探讨豚鼠眼球形觉剥夺后恢复期的生物学参数变化规律。方法普通级2～3周龄豚鼠30只,随机分为两组:①实验组:20只,右眼采用半透明眼罩遮盖进行形觉剥夺4周,随后去遮盖3周,左眼作为自身对照;②正常对照组:10只,双眼不进行任何干预,开放饲养7周。形觉剥夺前、形觉剥夺4周后及去遮盖后第2、6、10、14和21天,测量豚鼠双眼生物学参数:睫状肌麻痹后行带状光检影测量屈光度;A超测量前房深度、晶体厚度和眼轴长度,计算出玻璃体腔长度。结果经过4周形觉剥夺,实验组豚鼠右眼向近视漂移,屈光度为(-2.88±2.30)D,诱导了(-5.50±1.9)D相对近视。去遮盖后,豚鼠右眼重新正视化,屈光度恢复的快速期发生在6 d内,14 d时双眼屈光度差值差异无显著性(t=-2.049,P=0.080),为(-0.18±0.26)D;右眼玻璃体腔长度缩短,14 d时双眼玻璃体腔长度差值差异无显著性(t=1.652,P=0.14),为(0.0234±0.0400)mm;右眼眼轴长度缩短,14 d时双眼眼轴长度差值差差异无显著性(t=1.443,P=0.192),为(0.0183±0.0359)mm。与正常对照组右眼相比,去遮盖6 d,屈光度差异为(-0.48±0.36)D,差异无显著性(t=-1.325,P=0.206),而2 d时玻璃体腔和眼轴长度差异分别为(0.0961±0.0630)mm、(0.0621±0.0386)mm,差异无显著性(t=1.652,P=0.14;t=1.607,P=0.125)。结论 2～3周龄豚鼠去除形觉剥夺后可以重新进行正视化,伴随玻璃体腔和眼轴长度缩短;去遮盖6 d内为眼生物学参数恢复的主要时期。
Objective To investigate the changes of ocular biometric parameters during the recovery from form- deprivation myopia in guinea pigs. Methods Thirty guinea pigs, aged 2-3 weeks, were randomly divided into 2 groups： experimental group （n =20） and normal control group （n = 10）. The right eyes of guinea pigs in the experimental group were form-deprived with semi-transparent membranes for 4 weeks and subsequently allowed unrestricted vision for 3 weeks. The left eyes remained untreated as contralateral control eyes. The guinea pigs in the normal control group were raised with both eyes open for 7 weeks. Ocular biometfic parameters of the guinea pigs were measured before and 4 weeks after form- deprivation, and 2, 6, 10, 14, and 21 days after re-exposure. Refraction was measured by retinoscopy after cycloplegia. Axial length, anterior chamber depth and lens thickness were measured by A-scan ultrasound, and the vitreous chamber depth was calculated. Results After 4 weeks of treatment, the right eyes of guinea pigs in the experimentM group had my- opic shift with refraction of （ - 2. 88 ± 2. 30） D, and induced a relative myopia （ - 5.50 ±1.9） D. After re-exposure, theright eyes of guinea pigs in the experimental group experienced re-normalization, and the refraction was rapidly recovered in the initial 6 days. The refractive difference was （ -0. 18 ±0. 26） D, vitreous chamber depth difference was （0. 012 5 ± 0. 045 8） mm, and axial length difference was （0.018 3 ±0.035 9） mm between both eyes at 14 days after re-exposure, and were statistically non-significant （ t = - 2.049, P = 0. 080 ; t = 0. 610, P = 0. 575 ; t = 1. 443, P = 0. 192）. Compared with the right eyes of guinea pigs in the normal control group, the refractive difference （ - 0.48 ~ 0. 36） D was non-signifi- cant （t = - 1. 325 ,P = 0. 206） at 6 days after re-exposure, while the vitreous chamber depth difference was （0.0961 ± 0. 0630） mm and the axial length difference was （0. 062 1 ± 0. 038 6 ） mm, statistically non-significant （t = 1. 652, P = 0. 14;t = 1. 607,P = 0. 125）at 2 days after re-exposure. Conclusions Guinea pigs aged from 2 to 3 weeks with form-dep- rivation can experience re-normalization after re-exposure, along with a shortening of the vitreous chamber depth and axial length. The main recovery stage of ocular biometric parameters is in the initial 6 days after re-exposure.
出处
《中国实验动物学报》
CAS
CSCD
2012年第6期51-56,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
上海市金山区卫生局基金(编号:2011-06)
关键词
豚鼠
形觉剥夺
近视
恢复期
正视化
Guinea pig
Form deprivation
Myopia
Recovery
Normalization
